Comparison of methods to assay ammonia in bacterial suspensions

• Type: Article in International Scientific Journal
• Date: 1994

Authors

cabral, jps (Author) FCUP

Journal

Title: Journal of Microbiological Methods

Vol. 19

Pages: 207-213

ISSN: 0167-7012

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 2 Citations

Scopus - 3 Citations

Scientific classification

FOS: Natural sciences > Biological sciences

Other information

Authenticus ID: P-00H-6E3

DOI: 10.1016/0167-7012(94)90071-x

Abstract (EN): In the determination of the concentration of ammonia in the filtrates of Pseudomonas syringae cell suspensions, similar results were obtained using the indophenol method, a gas-sensing membrane electrode, and the L-glutamic dehydrogenase assay. The intracellular ammonia concentration was determined using the electrode and estimated to be 17 mM, corresponding to approximately 1/4 of potassium concentration. The results suggest that ammonium is one of the major intracellular cations in P. syringae cells.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 7