Abstract (EN):
Firefly oxyluciferin (2-(6'-hydroxybenzothiazolyl)-4-hydroxythiazole) was chemically synthesized and characterized by means of C-13 and H-1 NMR, UV-vis spectrometry and RP-HPLC using different pH elution conditions. One of the chromatographic peaks observed in luciferase-catalyzed reaction mixtures was identified Lis corresponding to oxyluciferin.
Language:
English
Type (Professor's evaluation):
Scientific
No. of pages:
4