In vitro and in vivo effect of fluoxetine on the permeability of H-3-serotonin across rat intestine

• Type: Article in International Scientific Journal
• Date: 2004

Authors

martel, f (Author) FMUP; monteiro, r (Author) FCNAUP; lemos, c (Author) Other; vieira-coelho, ma (Author) FMUP

Journal

Title: Canadian Journal of Physiology and Pharmacology

Vol. 82

Pages: 940-950

ISSN: 0008-4212

Publisher: NRC Research Press

Indexing

ISI Web of Knowledge - 7 Citations

Scopus - 8 Citations

Scientific classification

FOS: Medical and Health sciences > Basic medicine

Other information

Authenticus ID: P-000-7T8

DOI: 10.1139/y04-083

Abstract (EN): The aim of this work was to characterize the mucosal-to-serosal (apical to basolateral; AP-BL) and serosal-to-mucosal (basolateral to apical; BL-AP) transport of serotonin (5-HT) across rat jejunum, ileum, and colon, and to determine the influence of serotonin neuronal transporter inhibitors on this transport. The AP-BL apparent permeability (P-app) of H-3-5-HT increased in the order colon = jejunum < ileum, and the BL-AP P-app of H-3-5-HT increased in the order colon < jejunum = ileum. In vitro, neither fluoxetine (0.02 or 0.2 mumol/L) nor desipramine (0.4 or 4 mumol/L) had a significant effect upon the AP-BL or BL-AP P-app of H-3-5-HT in any of the intestinal regions. However, fluoxetine (0.2 mumol/L) decreased the accumulation of H-3-5-HT in the ileum (to 65% of control) in the BL-AP experiments. In vivo, chronic fluoxetine (10 mg/kg daily administered orally for 15 days), as assessed in the ileum, significantly increased (to +/-180% of control levels) the BL-AP P-app of H-3-5-HT and tended to increase the AP-BL P-app of H-3-5-HT. In conclusion, the increase in the P-app of H-3-5-HT after chronic administration of fluoxetine suggests that this treatment is able to increase the extracellular concentration of H-3-5-HT at the intestinal level.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 11