Effect of lifestyle on age-related mitochondrial protein oxidation in mice cardiac muscle

• Type: Article in International Scientific Journal
• Date: 2012

Authors

padrao, ai (Author) Other; ferreira, r (Author) Other; vitorino, r (Author) Other; alves, rmp (Author) Other; figueiredo, p (Author) Other; duarte, ja (Author) FADEUP; amado, f (Author) Other

Journal

Title: European Journal of Applied Physiology

Vol. 112 No. 9

Pages: 1467-1474

ISSN: 1439-6319

Publisher: Springer Nature

Indexing

ISI Web of Knowledge - 15 Citations

Scopus - 18 Citations

Scientific classification

FOS: Medical and Health sciences > Basic medicine

Other information

Authenticus ID: P-002-BTV

DOI: 10.1007/s00421-011-2100-3

Abstract (EN): This study investigated the influence of lifestyle on aging-related changes in cardiac proteins' oxidative modifications profile. Thirty C57BL/6 strain mice (2 months) were randomly divided into three groups (young Y, old sedentary S, and old active A). The S and A mice were individually placed into standard cages and in cages with running wheels, respectively, for 23 months. Upon killing, heart mitochondrial fractions were obtained for the evaluation of general proteins oxidative modifications profile, the identification of preferential protein targets, and oxidative phosphorylation (OXPHOS) activity. We observed age-related cardiac muscle impairment, evidenced by decreased OXPHOS activity, paralleled by an increased protein susceptibility to carbonylation and nitration. Among the main targets to these posttranslational modifications we found mitochondrial proteins, mainly from OXPHOS complexes, MnSOD and enzymes from lipid metabolism. Lifelong sedentary behavior exacerbated the nitrative damage of mitochondrial proteins, paralleled by a statistically significant decrease of respiratory chain complexes II and III activities. In overall, our results highlight the determinant role of aging in cardiac muscle impairment, which is worsened by a sedentary lifestyle.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 8