Discriminating adenocarcinoma from normal colonic mucosa through deconvolution of Raman spectra

• Type: Article in International Scientific Journal
• Date: 2011

Authors

Lopes, PC (Author) Other; Moreira, JA (Author) FCUP; Almeida, A (Author) Other; Esteves, A (Author) Other; Gregora, I (Author) Other; Ledinsky, M (Author) Other; Lopes, JM (Author) Other; Henrique, R (Author) Other; Oliveira, A (Author) Other

Journal

Title: Journal of Biomedical Optics

Vol. 16

Final page: 127001

ISSN: 1083-3668

Publisher: SPIE

Indexing

ISI Web of Knowledge - 23 Citations

Scopus - 26 Citations

Other information

Authenticus ID: P-002-HVD

DOI: 10.1117/1.3658756

Abstract (EN): In this work, we considered the feasibility of Raman spectroscopy for discriminating between adenocarcinomatous and normal mucosa] formalin-fixed colonic tissues. Unlike earlier studies in colorectal cancer, a spectral deconvolution model was implemented to derive spectral information. Eleven samples of human colon were used, and 55 spectra were analyzed. Each spectrum was resolved into 25 bands from 975 to 1 720 cm(-1), where modes of proteins, lipids, and nucleic acids are observed. From a comparative study of band intensities, those presenting higher differences between tissue types were correlated to biochemical assignments. Results from fitting procedure were further used as inputs for linear discriminant analysis, where combinations of band intensities and intensity ratios were tested, yielding accuracies up to 81%. This analysis yields objective discriminating parameters after fitting optimization. The bands with higher diagnosis relevance detected by spectra deconvolution enable to confine the study to some spectral regions instead of broader ranges. A critical view upon limitations of this approach is presented, along with a comparison of our results to earlier ones obtained in fresh colonic tissues. This enabled to assess the effect of formal in fixation in colonic tissues, and determine its relevance in the present analysis. (C) 2011 Society of Photo-Optical Instrumentation Engineers (SPIE). [DOI: 10.1117/1.3658756]

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 12