Distinct modulation of alkaline phosphatase isoenzymes by 17 beta-estradiol and xanthohumol in breast cancer MCF-7 cells

• Type: Article in International Scientific Journal
• Date: 2007

Authors

Susana Guerreiro (Author) Other; Rosario Monteiro (Author) FCNAUP; Maria Joao Martins (Author) FMUP; Conceicao Calhau (Author) FMUP; Isabel Azevedo (Author) Other; Raquel Soares (Author) FMUP

Journal

Title: Clinical Biochemistry

Vol. 40

Pages: 268-273

ISSN: 0009-9120

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 36 Citations

Scopus - 39 Citations

Scientific classification

FOS: Engineering and technology > Medical engineering

Other information

Authenticus ID: P-004-BY9

DOI: 10.1016/j.clinbiochem.2006.09.012

Abstract (EN): Objectives: To examine the effect of 17 beta-estradiol and xanthohumol in alkaline phosphatase (ALP) expression and activity in breast cancer MCF-7 cells. Design and methods: ALP isoenzytnes expression was evaluated by RT-PCR and Western blotting. ALP activity was measured by spectrophotometry. Cell proliferation and apoptosis were examined by MTT and immunostaining for K167 and TUNEL, respectively. Results: ALP isoenzymes expression and activity were decreased by 1 nM 17 beta-estradiol. Pure estrogenic antagonist (ICI 182,780) reversed 17 beta-estradiol-inhibiting effect in TNS-ALP expression. RNA and protein expression of IALP, but not TNS-ALP, was also decreased by incubation with 10 mu M xanthohumol (IC50) and was accompanied by a significant reduction in ALP activity. Treatment with 17 beta-estradiol enhanced cell proliferation and decreased apoptosis. Conversely, xanthohumol incubation inhibited cell viability and apoptosis. Conclusion: Estrogens and xanthohumol differently modulate ALP isoenzymes. ALP loss associated with increased cell proliferation. Modulation of this enzyme by 17 beta-estradiol and xanthohumol might provide therapeutic strategies against hormone-dependent breast cancer.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 6