Differential activation of protein kinase C isoforms by euxanthone, revealed by an in vivo yeast phenotypic assay

• Type: Article in International Scientific Journal
• Date: 2002

Authors

Saraiva, L (Author) FFUP; Fresco, P (Author) FFUP; Pinto, E (Author) FFUP; Kijjoa, A (Author) ICBAS; Gonzlaez, MJ (Author) Other; Goncalves, J (Author) FFUP

Journal

Title: Planta Medica

Vol. 68

Pages: 1039-1041

ISSN: 0032-0943

Publisher: Thieme

Indexing

ISI Web of Knowledge - 13 Citations

Scopus - 14 Citations

Scientific classification

FOS: Medical and Health sciences > Basic medicine

Other information

Authenticus ID: P-000-MMB

DOI: 10.1055/s-2002-35655

Abstract (EN): The protein kinase C (PKC) modulatory effects of euxanthone, isolated from the wood of Cratoxylum maingayi, on isoforms alpha, betaI, delta, eta and xi were characterised using an alternative in vivo yeast phenotypic assay. The present study shows that euxanthone can activate isoforms alpha, betaI, delta, eta and xi, being more effective on PKC-betaI, -delta, -eta and -xi than the established PKC activators used (the phorbol ester PMA and arachiclonic acid for PKC-xi). Furthermore, euxanthone presents differences on its potency towards individual PKC isoforms, showing a remarkable selectivity for PKC-xi. These results can help to clarify the molecular basis of the euxanthone-mediated effects.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 3