In organello assembly of respiratory-chain complex I: Primary structure of the 14.8 kDa subunit of Neurospora crassa complex I

• Type: Article in International Scientific Journal
• Date: 1994

Authors

azevedo, je (Author) ICBAS; eckerskorn, c (Author) Other; werner, s (Author) Other

Journal

Title: Biochemical Journal

Vol. 299

Pages: 297-302

ISSN: 0264-6021

Publisher: PORTLAND PRESS LTD

Indexing

ISI Web of Knowledge

Scopus - 2 Citations

Other information

Authenticus ID: P-007-682

Abstract (EN): A cDNA encoding the 14.8 kDa subunit of complex I from Neurospora crassa was cloned and sequenced. The deduced primary structure of this subunit reveals a predominantly hydrophilic protein containing no obvious membrane-spanning domain. In agreement with this characteristic, we have localized the 14.8 kDa subunit in the peripheral arm of the enzyme. The 14.8 kDa subunit was found to be conserved in mammalian complex I. The conservation of this subunit in such distantly related organisms suggests that the 14.8 kDa subunit is an important component of complex I. We have used an in organello system to study the biosynthetic pathway of this subunit. The 14.8 kDa polypeptide could be efficiently imported into isolated mitochondria. Furthermore, a fraction of the in-vitro-imported subunit was found to assemble in complex I. This is the first time that assembly in complex I of an in-vitro-synthesized subunit is demonstrated.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 6