First Portuguese isolate of Neospora caninum from an aborted fetus from a dairy herd with endemic neosporosis

• Type: Article in International Scientific Journal
• Date: 2002

Authors

canada, n (Author) ICBAS; meireles, cs (Author) Other; rocha, a (Author) ICBAS; sousa, s (Author) Other; thompson, g (Author) ICBAS; dubey, jp (Author) Other; romand, s (Author) Other; thulliez, p (Author) Other; da costa, jmc (Author) Other

Journal

Title: Veterinary Parasitology

Vol. 110

Pages: 11-15

ISSN: 0304-4017

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 14 Citations

Scopus - 15 Citations

Other information

Authenticus ID: P-000-M2K

DOI: 10.1016/S0304-4017(02)00333-3

Abstract (EN): Neospora caninum was isolated from the brain of an aborted 4-month-old fetus from a dairy cow herd with endemic neosporosis in Porto, Portugal. The fetal brain homogenate was inoculated interperitoneally first into outbred Swiss Webster mice given dexamethasone and then the peritoneal exudates from these mice was co-inoculated with mouse sarcoma cells in the peritoneal cavity of mice given dexamethasone. N. caninum tachyzoites were seen in peritoneal exudate of the second passage. Tachyzoites from the peritoneal exudate reacted positively with anti-N. caninum antibodies and not with anti-Toxoplasma gondii antibodies and contained N. caninum specific DNA. This Portuguese isolate of N. caninum has been successfully maintained in cell culture. The dam of the aborted fetus had an antibody titer of 1: 10240 in the Neospora agglutination test (NAT). Antibodies to N. caninum were found in 76 of 106 cows from this herd in titers of 1:40 in 31, 1:80 in 22, greater than or equal to 1: 160 or more in 23 in the Neospora agglutination test. This is the first isolation of a viable N. caninum-like parasite from any host in Portugal. (C) 2002 Elsevier Science B.V. All rights reserved.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 5