Effects of natural prenylated flavones in the phenotypical ER(+) MCF-7 and ER(-) MDA-MB-231 human breast cancer cells

• Type: Article in International Scientific Journal
• Date: 2006

Authors

Pedro, M (Author) Other; Lourenco, CF (Author) Other; Cidade, H (Author) FFUP; Kijjoa, A (Author) ICBAS; Pinto, M (Author) FFUP; Nascimento, MSJ (Author) FFUP

Journal

Title: Toxicology Letters

Vol. 164 No. 1

Pages: 24-36

ISSN: 0378-4274

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 25 Citations

Scopus - 28 Citations

Scientific classification

FOS: Medical and Health sciences > Basic medicine

Other information

Authenticus ID: P-004-K5D

DOI: 10.1016/j.toxlet.2005.11.007

Abstract (EN): The effect of seven natural prenylated flavones in DNA synthesis of two human breast cancer cell lines, the estrogen-dependent ER (+) MCF-7 and the estrogen-independent ER (-) MDA-MB-231 cells, was evaluated. Flavones with an isopentenyl group at C-8 and a ring linking C-3 and C-2 ' presented a biphasic effect in DNA synthesis of ER (+) MCF-7 and displayed a stimulation at low concentrations (0.02-0.78 mu M) whilst at higher concentrations (> 3.12 mu M) inhibition was observed. No stimulation was observed in ER (-) MDA-MB-231. In contrast, all the flavones exhibited an antiproliferative effect in both ER (-) and ER (+). Curiously, the inhibition of DNA synthesis was accompanied by a high capacity of these cells to reduce MTT, which was concurrent with the appearance of an intense intracytoplasmic vacuolization. The accumulation of the formazan product in these vacuoles could justify the enhancements of MTT reduction. The characterization of these vacuoles with the autophagic marker monodansylcadaverine (MDC) is consistent with autophagic vacuoles, which led to the suggestion that these flavones could induce autophagy in both ER (+) and ER (-) breast cancer cell lines.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 13