IMMUNOLOGICAL CHARACTERIZATION OF HYDROGENASES IN THE NITROGEN-FIXING CYANOBACTERIUM NOSTOC SP STRAIN PCC-73102

• Type: Article in International Scientific Journal
• Date: 1995

Authors

Paula Tamagnini (Author) Other; Fredrik Oxelfelt (Author) Other; Roberto Salema (Author) FCUP; Peter Lindblad (Author) Other

Journal

Title: Current Microbiology

Vol. 31

Pages: 102-107

ISSN: 0343-8651

Publisher: Springer Nature

Indexing

ISI Web of Knowledge - 14 Citations

Scopus - 11 Citations

Other information

Authenticus ID: P-001-GH9

DOI: 10.1007/bf00294284

Abstract (EN): N-2-fixing Nostoc sp. strain PCC 73102 was examined for the presence of hydrogenases. Native-PAGE/immunoblots demonstrated that two proteins with molecular masses of approximately 200 kDa and 215 kDa are immunologically related to hydrogenases purified from Bradyrhizobium japonicum, Azotobacter vinelandii, Methanosarcina barkeri, and Thiocapsa roseopersicina. SDS-PAGE/immunoblots showed that one polypeptide, with a molecular mass of about 58 kDa, is immunologically related to the hydrogenases purified from all the microorganisms mentioned above. In addition, two polypeptides, with molecular masses of approximately 34 and 70 kDa, are immunologically related to the hydrogenases purified from T. roseopersicina and M. barkeri respectively. Immunogold/transmission electron microscopy showed that the hydrogenase proteins are present in both the heterocysts and the vegetative cells.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 6