Gold-silver-alloy nanoprobes for one-pot multiplex DNA detection

• Type: Article in International Scientific Journal
• Date: 2010

Authors

Doria, G (Author) Other; Larguinho, M (Author) Other; Dias, JT (Author) Other; Pereira, E (Author) FCUP; Franco, R (Author) Other; Baptista, PV (Author) Other

Journal

Title: Nanotechnology

Vol. 21 No. 30

Final page: 255101

ISSN: 0957-4484

Publisher: Institute of Physics Publishing

Indexing

ISI Web of Knowledge - 30 Citations

Scopus - 33 Citations

Scientific classification

FOS: Engineering and technology > Materials engineering

Other information

Authenticus ID: P-003-5MC

DOI: 10.1088/0957-4484/21/25/255101

Abstract (EN): A specific colorimetric DNA detection method based on oligonucleotide functionalized gold-silver-alloy nanoparticles (AuAg-alloy-nanoprobes) is presented. The AuAg-alloy-nanoprobes were then used for the specific detection of a DNA sequence from TP53-a gene involved in cancer development. The AuAg-alloy-nanoprobes were then used in combination with Au-nanoprobes for a one-pot dual-colour detection strategy that allowed for the simultaneous differential detection of two distinct target sequences. This system poses an unprecedented opportunity to explore the combined use of metal nanoparticles with different composition towards the development of a multiplex one-pot colorimetric assay for DNA detection.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 5