Nickel induces oxidative burst, NF-kappa B activation and interleukin-8 production in human neutrophils

• Type: Article in International Scientific Journal
• Date: 2010

Authors

Marisa Freitas (Author) Other; Ana Gomes (Author) Other; Graca Porto (Author) ICBAS; Eduarda Fernandes (Author) FFUP

Journal

Title: Journal of Biological Inorganic Chemistry

Vol. 15 No. 6

Pages: 1275-1283

ISSN: 0949-8257

Publisher: Springer Nature

Indexing

ISI Web of Knowledge - 25 Citations

Scopus - 29 Citations

Scientific classification

FOS: Natural sciences > Chemical sciences

Other information

Authenticus ID: P-003-1F1

DOI: 10.1007/s00775-010-0685-3

Abstract (EN): Many lines of evidence have suggested that oxidative stress and inflammation play a pivotal role in the toxicity of nickel salts Considering that neutrophils are active participants in inflammatory processes, namely by producing high amounts of reactive oxygen species, the aim of the present study was to evaluate the putative activation of human neutrophils' oxidative burst by nickel Subsequently, the influence of nickel in the pathways leading to NADPH oxidation in neutrophils was evaluated by measuring protein kinase C (PKC) activation The effects of nickel on neutrophils' nuclear factor kappa B (NF-kappa B) activation and on the production of the proinflammatory mediators interleukin (IL)-1 beta, IL-6, IL-8 and tumour necrosis factor a were also evaluated The results obtained showed that nickel, at concentrations that may be attained in vivo, stimulates the production of superoxide radical (O-2(-)), hydrogen peroxide (H2O2), and hypochlorous acid (HOCI) in human neutrophils in vitro, via activation of PKC In addition, nickel was shown to activate NF-kappa B and to induce the production of IL-8 in these cells These observations indicate that the sustained activation of human neutrophils by nickel may contribute for the long-term adverse effects on human health mediated by this metal

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 9