Modulation of MPP+ uptake by procyanidins in Caco-2 cells: Involvement of oxidation/reduction reactions

• Type: Article in International Scientific Journal
• Date: 2006

Authors

Faria, A (Author) FMUP; Mateus, N (Author) FCUP; de Freitas, V (Author) FCUP; Calhau, C (Author) FMUP

Journal

Title: FEBS Letters

Vol. 580

Pages: 155-160

ISSN: 0014-5793

Publisher: Wiley-Blackwell

Indexing

ISI Web of Knowledge - 22 Citations

Scopus - 27 Citations

Scientific classification

FOS: Natural sciences > Biological sciences

Other information

Authenticus ID: P-004-NQ9

DOI: 10.1016/j.febslet.2005.11.068

Abstract (EN): It is becoming increasingly evident that the absorption of certain nutrients and drugs and their effects are largely influenced by the concomitant ingestion of other substances. As various xeno- and endobiotics belong to the class of organic cations, the aim of this work was to study the modulation of the intestinal apical uptake of organic cations by diet procyanidins. Five procyanidin fractions with different structural complexity were obtained after fractionation of a grape seed extract. The effect of these compounds on 1-methyl-4-phenylpyridinium (MPP+) uptake was evaluated in Caco-2 cells. Apical uptake of H-3-MPP+ by Caco-2 cells was increased by a 60 min exposure to 600 mu g ml(-1) of procyanidin fractions, that increase being positively related with procyanidins structural complexity. It was verified that H-3-MPP+ uptake increased with preincubation time. It was speculated that procyanidins were oxidized during preincubation, this change could interfered with transport activity. Tested oxidizing agents showed that the redox state of the transporter could affect its activity. Additionally, trans-stimulation experiments showed that catechin and fraction I (the simpler fraction) can use the same transporter as MPP+. The results are compatible with the hypothesis of these compounds being competitive inhibitors of MPP+ transport. In conclusion, procyanidins are capable to modulate MPP+ apical uptake in Caco-2 cells, this transport being most probably modulated through oxidation-reduction phenomena. Interactions between these compounds and drugs present in the diet may affect their absorption and bioavailability. Both the concentration and complexity of the procyanidin compounds should be taken into account in medical practice.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 6