Primary structure of a ferredoxin-like iron-sulfur subunit of complex I from Neurospora crassa

• Type: Article in International Scientific Journal
• Date: 1996

Authors

duarte, m (Author) Other; finel, m (Author) Other; videira, a (Author) ICBAS

Journal

Title: Biochimica et Biophysica Acta - Bioenergetics

Vol. 1275

Pages: 151-153

ISSN: 0005-2728

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 16 Citations

Scopus - 16 Citations

Other information

Authenticus ID: P-001-DZV

DOI: 10.1016/0005-2728(96)00033-3

Abstract (EN): We have isolated cDNA clones encoding an iron-sulfur polypeptide subunit of the mitochondrial complex I of Neurospora crassa. The fungal cDNA library was screened by hybridisation with an heterologous probe from Paracoccus denitrificans. The DNA sequence of relevant isolates was determined and revealed an open reading frame encoding a precursor protein of 219 amino acid residues. The gene product is a ferredoxin-like protein that contains two cysteine-rich motives that may each bind a tetranuclear iron-sulfur cluster. The primary structure of the protein is highly homologous to the 23 kDa iron-sulfur subunit of complex I from bovine and P. denitrificans. Interestingly, an alanine residue within the second cluster-binding motif, which is conserved in complex I but replaced by tyrosine in similar chloroplast genes, is substituted for serine in N. crassa.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 3