Primary structure and characterisation of a 64 kDa NADH dehydrogenase from the inner membrane of Neurospora crassa mitochondria

• Type: Article in International Scientific Journal
• Date: 1999

Authors

melo, amp (Author) Other; duarte, m (Author) Other; videira, a (Author) ICBAS

Journal

Title: Biochimica et Biophysica Acta - Bioenergetics

Vol. 1412

Pages: 282-287

ISSN: 0005-2728

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 36 Citations

Scopus - 36 Citations

Other information

Authenticus ID: P-001-3NG

DOI: 10.1016/S0005-2728(99)00072-9

Abstract (EN): A cDNA clone encoding a mitochondrial NADH dehydrogenase from Neurospora cia,rsa was sequenced. The total DNA sequence encompasses 2570 base pairs and contains an open reading frame of 2019 base pairs coding for a precursor polypeptide of 673 amino acid residues. The protein is encoded by a single-copy gene located to the right side of the centromere in linkage group IV of the fungal genome. The N-terminus of the precursor protein has characteristics of a mitochondrial targeting pre-sequence. The protein displays homology with mitochondrial NADH dehydrogenases from yeast. In contrast to these polypeptides, however, analysis of its primary structure revealed that it contains a well-conserved calcium-binding domain. Rabbit antiserum against the protein expressed in an heterologous system recognises a mitochondrial protein of N. crassa with an apparent molecular mass of 64 kDa. Analysis of the fungal mitochondria by swelling, digitonin fractionation and alkaline treatment indicate that the protein is located in the inner membrane of the organelles, possibly facing the matrix side. (C) 1999 Elsevier Science B.V. All rights reserved.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 6