The 9.8 kDa subunit of complex I, related to bacterial Na+-translocating NADH dehydrogenases, is required for enzyme assembly and function in Neurospora crassa

• Type: Article in International Scientific Journal
• Date: 2003

Authors

marques, i (Author) Other; duarte, m (Author) ICBAS; videira, a (Author) ICBAS

Journal

Title: Journal of Molecular Biology

Vol. 329

Pages: 283-290

ISSN: 0022-2836

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 15 Citations

Scopus - 16 Citations

Other information

Authenticus ID: P-000-GKN

DOI: 10.1016/S0022-2836(03)00443-1

Abstract (EN): A nuclear gene encoding a 9.8 kDa. subunit of complex 1, the homologue of mammalian MWFE protein, was identified in the genome of Neurospora crassa. The gene was cloned and inactivated in vivo by the generation of repeat-induced point mutations. Fungal mutant strains lacking the 9.8 kDa. polypeptide were subsequently isolated. Analyses of mitochondrial proteins from mutant nuo9.8 indicate that the membrane and peripheral arms of complex I fail to assemble. Respiration of mutant mitochondria. on matrix NADH is rotenone-insensitive, confirming that the 9.8 kDa. protein is required for the assembly and activity of complex I. We found a similarity between the MWFE homologues and the C-terminal part of the nqrA subunit of bacterial Na+-translocating NADH:quinone oxidoreductases (Na+-NQR), suggesting a link between proton-pumping and sodium-pumping NADH dehydrogenases. (C) 2003 Elsevier Science Ltd. All rights reserved.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 8