Murine model of neonatal and infantile pulmonary hypertension induced by monocrotaline: morphometric and hemodynamic evaluation.

• Type: Summary of Presentation in an International Conference
• Date: 2006

Authors

Henriques-Coelho T (Author) FMUP; Santos M (Author) FMUP; Oliveira SM (Author) FMUP; Gonçalves D (Author) Other; Correia-Pinto J (Author) FMUP; Leite-Moreira AF (Author) FMUP

Conference proceedings International

Title: European Heart Journal

Initial page: 618

World Congress of Cardiology

Barcelona, SPAIN, 02 a 06 de Setembro de 2006

Scientific classification

FOS: Medical and Health sciences > Other medical sciences

Other information

Resumo (PT): Pulmonary hypertension (PH) is characterized by structural changes of the pulmonary vessels as well as right ventricular (RV) hypertrophy. The reversibility of pulmonary vascular remodeling is possible only at paediatric ages. The monocrotaline (MCT)-induced PH model is well defined for adult animals and mimics human PH. The aim of the study is to establish neonatal and infantile HTP models by MCT administration and to evaluate morphometric and haemodynamic parameters. Protocol 1. Wistar rats 3- and 8-day-old were injected with different doses of MCT: a) 60 mg/kg, n=35; b) 50mg/kg, n=31; c) 30mg/kg, n=40. Protocol 2. Wistar rats with 3 (neonatal) or 8-day-old (infantile) randomly received a subcutaneous injection of MCT 30mg/kg (MCT-Neo, n=10; MCT-Inf, n=10) or an equal volume of vehicle (Ctrl-Neo, n=10; Ctrl-Inf, n=10). At day 21 after injection, RV and left ventricular (LV) peak systolic (RVPmax) and end-diastolic (RVEDP) pressures, peak rates of ventricular pressure rise (dP/dtmax) and relaxation rate (Tau RV) were recorded. At the end, heart and lungs were excised and weighted. Protocol 1. Survival rates at day 21: MCT60=0%; MCT50=0%; MCT30=85%. Protocol 2. Results expressed as means±SE and summarized in table. P<0.05: a vs Ctrl. Protocol 2 - Results Ctrl-Neo MCT-Neo Ctrl-Inf MCT-Inf Body Weight, g 46±1 47±2 80±1 58±3 a RV/LV g/g 0.29±0.02 0.41±0.03 a 0.28±0.02 0.40±0.02 a Lung, g 0.404±0.02 0.478±0.02 0.680±0.28 0.656±0.83 RVPmax, mmHg 18.3±1.9 41.9±1.3 a 19.6±2.8 37.1±4.7 a RVEDP, mmHg 1.1±0.4 2.3±0.5 a 0.4±0.7 2.9±1.4 a dP/dtmax RV, mmHg/s 657±157 1094±106 a 663±74 1210±258 a Tau RV, ms 20±1 31±4 a 21±5 33±9 a We characterized haemodynamics of healthy and PH Wistar rats during the childhood period. We established the survival until 3 weeks after the injection of MCT 30mg/kg. We documented, in both neonatal and infantile groups, prominent RV hypertrophy, pulmonary hypertension and diastolic dysfunction. No differences were found in LV data. This model will allow to study the similarities and differences with the adult model and to test the effects of new drugs in neonatal and infantile PH.

Language: English

Type (Professor's evaluation): Scientific

Notes: XVth World Congress of Cardiology, published in journal, European Heart Journal. 2006; Vol.27(Suppl.1):618-618.