Differential expression of GABA(B(1b)) receptor mRNA in the thalamus of normal and monoarthritic animals

• Type: Article in International Scientific Journal
• Date: 2004

Authors

Ferreira-Gomes J. (Author) FCNAUP; Neto F.L. (Author) FMUP; Castro-Lopes J.M. (Author) FMUP

Journal

Title: Biochemical Pharmacology

Vol. 68 No. 8

Pages: 1603-1611

ISSN: 0006-2952

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 12 Citations

Scopus - 17 Citations

Scientific classification

CORDIS: Health sciences > Neuroscience > Neurobiology

Other information

Authenticus ID: P-000-83B

DOI: 10.1016/j.bcp.2004.07.018

Abstract (EN): GABA(B) receptors have been implicated in the plastic changes occurring in the spinal cord during the development of chronic inflammatory pain. In this study, we evaluated whether the expression of GABA(B(1b)) receptor mRNA is regulated supraspinally, namely in the thalamus, as part of the response to chronically enhanced noxious input arising from experimental monoarthritis (MA). In situ hybridization with [S-35] -labelled oligonucleotide probes was performed in sections of control, 2, 4, 7 and 14 days MA rats' brains (n = 6/group). The distribution of GABA(B(1b)) mPNA was determined bilaterally in the ventrobasal complex (VB), posterior (Po), centromedial/centrolateral (CM/CL) and reticular (Rt) thalamic nuclei. The amount of GABA(B(1b)) mRNA was expressed as times fold of background values. In normal animals, values of mRNA expression were very similar in VB, Po and CM/CL, ranging from 2.2 +/- 0.2 to 2.7 +/- 0.4 (mean +/- S.E.M.) times higher than background levels. No expression of GABA(B(1b)) mRNA was found in the Rt of control or MA animals. A significant decrease of 26% at 4 days, and 37% at 7 days of MA, was observed in the VB contralateral to the affected joint. On the contrary, in the Po there was a significant bilateral increase at 2 days (38% contralaterally, 25% ipsilaterally), returning to basal levels at 4 days MA. No significant changes were observed in CM/CL. These results suggest that the expression of GABA(B(1b)) in the VB and Po is regulated by noxious input, and might contribute to the functional changes that occur in the thalamus during chronic inflammatory pain.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 9