Remote myocardium gene expression after 30 and 120 min of ischaemia in the rat

• Type: Article in International Scientific Journal
• Date: 2006

Authors

Guerra MS (Author) Other; Roncon-Albuquerque R Jr (Author) FMUP; Lourenço AP (Author) FMUP; Falcão-Pires I (Author) FMUP; Cibrão-Coutinho P (Author) Other; Leite Moreira AF (Author) FMUP

Journal

Title: Experimental Physiology

Vol. 91 No. 2

Pages: 473-480

ISSN: 0958-0670

Publisher: Wiley-Blackwell

Indexing

ISI Web of Knowledge - 12 Citations

Scopus - 13 Citations

Scientific classification

FOS: Medical and Health sciences > Other medical sciences

Other information

Authenticus ID: P-004-MYD

DOI: 10.1113/expphysiol.2005.032557

Abstract (EN): The aim of the present study was to investigate how early the onset of ischaemia-induced changes in gene expression is in remote myocardium, and whether these changes would be different for left and right ventricles. Wistar rats (n=27) were randomly assigned to left coronary artery (LCA) ligation for 30 or 120 min and sham groups. Evans Blue infusion revealed antero-apical left ventricle (LV) and left intraventricular (IV) septal ischaemia (35.5 +/- 0.6% of LV mass). LCA ligation induced transient LV systolic dysfunction and sustained biventricular slowing of relaxation. Regarding mRNA levels, type B natriuretic peptide (BNP) was upregulated in the LV at 30 (+370 +/- 191%) and 120 min (+221 +/- 112%), whilst in the right ventricle (RV) this was only significant at 120 min (+128 +/- 39%). Hipoxia-inducible factor 1 alpha and interleukin 6 overexpression positively correlated with BNP. Inducible NO synthase upregulation was present in both ventricles at 120 min (LV, +327 +/- 195%; RV, +311 +/- 122%), but only in the RV at 30 min (+256 +/- 88%). Insulin-like growth factor 1 increased in both ventricles at 30 (RV, +59 +/- 18%; LV, +567 +/- 192%) and 120 min (RV, +69 +/- 33%; LV, +120 +/- 24%). Prepro-endothelin-1 was upregulated in the RV at 120 min (+77 +/- 25%). Ca2+-handling proteins were selectively changed in the LV at 120 min (sarcoplasmic reticulum Ca2+ ATPase, 53 +/- 7%; phospholamban, +31 +/- 4%; Na+-Ca2+ exchanger, 31 +/- 6%), while Na+-H+ exchanger was altered only in the RV (-79 +/- 5%, 30 min; +155 +/- 70%, 120 min). Tumour necrosis factor-alpha and angiotensin converting enzyme were not significantly altered. A very rapid modulation of remote myocardium gene expression takes place during myocardial ischaemia, involving not only the LV but also the RV. These changes are different in the two ventricles and in the same direction as those observed in heart failure.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 8