Identification of cell-surface mannans in a virulent Helicobacter pylori strain

• Type: Article in International Scientific Journal
• Date: 2010

Authors

Jose A Ferreira (Author) Other; Nuno F Azevedo (Author) FEUP; Maria Joao Vieira (Author) Other; Ceu Figueiredo (Author) FMUP; Brian J Goodfellow (Author) Other; Mario A Monteiro (Author) Other; Manuel A Coimbra (Author) Other

Journal

Title: Carbohydrate Research

Vol. 345

Pages: 830-838

ISSN: 0008-6215

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 11 Citations

Scopus - 11 Citations

Scientific classification

FOS: Natural sciences > Chemical sciences

Other information

Authenticus ID: P-003-7EQ

DOI: 10.1016/j.carres.2010.01.022

Abstract (EN): With the intent of contributing to a carbohydrate-based vaccine against the gastroduodenal pathogen, Helicobacter pylori, we report here the structure of cell-surface mannans obtained from a virulent strain. Unlike other wild-type strains, this strain was found to express in good quantities this polysaccharide in vitro. Structural analysis revealed a branched mannan formed by a backbone of alpha-(1 -> 6)-linked mannopyranosyl residues with approximately 80% branching at the O-2 position. The branches were composed of O-2-linked Man residues in both alpha- and beta-configurations: ->[6)-alpha-D-Manp-(1 -> 6](n)-alpha-D-Manp-(1 -> 6 R1 -> 2 R = alpha-D-Man; ->[2)-alpha-D-Man-(1](n)-> 2)-alpha-D-Man; ->[2)-alpha-D-Man-(1]n ->? In addition, this strain also expressed cell-surface emblematic H. pylori lipopolysaccharides (LPS) containing partially fucosylated polyLacNAc O-chains. Affinity assays with polymyxin-B and concanavalin A revealed no association between the mannan and the LPS. The described mannans may be implicated in the mediation of host-microbial interactions and immunological modulation.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 9