A light- and electron-microscopic histopathological study of human bladder mucosa after intravesical resiniferatoxin application

• Type: Article in International Scientific Journal
• Date: 2001

Authors

Carlos Silva (Author) FMUP; António Avelino Silva (Author) FMUP; C. Souto Moura (Author) Other; Francisco Cruz (Author) FMUP

Journal

Title: BJU International

Vol. 88

Pages: 355-360

ISSN: 1464-4096

Publisher: Wiley-Blackwell

Indexing

ISI Web of Knowledge - 20 Citations

Scopus - 29 Citations

Scientific classification

FOS: Medical and Health sciences > Other medical sciences

Other information

Authenticus ID: P-000-TBH

DOI: 10.1046/j.1464-410x.2001.02339.x

Abstract (EN): Objective To determine the morphology of bladder mucosa and the integrity of its mucin coat in patients with detrusor hyper-reflexia treated with intravesical resiniferatoxin. Patients and methods Seven patients with detrusor hyper-reflexia were treated intravesically with resiniferatoxin dissolved in 10%, ethanol in saline (50 nmol/L solution in two and 100 nmol/L, in five). Patients were clinically evaluated by a voiding chart and filling cystometry before and 3 months after each resiniferatoxin application. In addition, they underwent cystoscopy and bladder biopsies at 22-33 months after the first instillation and at 7-23 months after the last one. Tissue samples for light microscopy were fixed in 4% paraformaldehyde, embedded in paraffin and stained with haematoxylincosin or periodic acid-Schiff reagent (PAS). Those for electron microscopy were fixed in 5% glutaraldehyde and embedded in resin. Results The resiniferatoxin instillation was not painful. Three months after treatment the mean voiding frequency decreased and live incontinent patients became continent. The maximum cystometric capacity increased in all patients; at cystoscopy the bladders appeared normal. On light microscopy the urothelium was of normal morphology and stained with PAS in the luminal cells and in the basement membrane. Mononuclear inflammatory cells were occasionally apparent in the lamina propria. On electron microscopy epithelial cells were visible in a thick basal lamina. Superficial cells had the usual irregular contour and contained numerous membrane-coated vesicles. In the lamina propria, unmyelinated axonal profiles with occasional varicosities Could be identified. Conclusions Intravesical resiniferatoxin improved urinary frequency, incontinence and bladder capacity in patients with detrusor hyper-reflexia, causing no morphological change in the bladder mucosa. The PAS reactivity of the carbohydrate moieties present in the mucin coat and the basement membrane was unchanged by resiniferatoxin.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 6