INVOLVEMENT OF TUBULAR SODIUM IN THE FORMATION OF DOPAMINE IN THE HUMAN RENAL-CORTEX

• Type: Article in International Scientific Journal
• Date: 1993

Authors

Soares-da-Silva P (Author) Other; Pestana M (Author) Other; Fernandes MH (Author) FMDUP

Journal

Title: Journal of the American Society of Nephrology

Vol. 3

Pages: 1591-1599

ISSN: 1046-6673

Publisher: American Society of Nephrology

Indexing

ISI Web of Knowledge - 47 Citations

Scientific classification

FOS: Medical and Health sciences > Other medical sciences

Other information

Authenticus ID: P-001-NAW

Abstract (EN): This study has examined the influence of sodium (0, 20, 40, 80, 120, and 160 mM) and ouabain (100, 500, and 1,000 muM), an inhibitor of the enzyme Na+-K+ ATPase, on the synthesis of dopamine in slices of human renal cortex loaded with exogenous L-dihydroxyphenylalanine (L-DOPA). The deamination of newly formed dopamine into 3,4-dihydroxyphenylacetic acid (DOPAC) was also examined. The formation of dopamine and its deamination to DOPAC in slices and homogenates of human renal cortex closely depended on the concentration Of L-DOPA added to the medium; in homogenates of renal cortex, the production of dopamine was found to be 74% of that occurring in the renal medulla. Decarboxylation Of L-DOPA was found saturable at 1,000 muM L-DOPA, which had V(max) and K(m) values for L-amino acid decarboxylase activity of, respectively, 5.8 +/-0.6 nmol/mg of protein per hour and 62 +/- 8 muM. The accumulation of newly formed dopamine and DOPAC in kidney slices loaded with L-DOPA (50 and 100 muM) was found to be partially dependent on the concentration of sodium in the medium; at 0 mM sodium, the synthesis of dopamine from L-DOPA was found to be half of that occurring at 160 mM sodium. A similar picture could be observed for DOPAC. The fractional rate of accumulation (k; mM sodium-1) at 50 and 100 muM L-DOPA was, respectively, 0.0016 +/-0.0002 and 0.0016 +/- 0.0005 for dopamine and 0.0018 +/- 0.0002 and 0.0021 +/- 0.0005 for DOPAC. In experiments performed in the presence of 120 and 160 mM sodium, ouabain (100, 500, and 1,000 muM) was found to inhibit the accumulation of newly formed dopamine and DOPAC (12 to 63% reduction; P < 0.05) in slices loaded with 50 muM L-DOPA. When the experiments were performed in the presence of 20 mM sodium in the incubation medium, the renal production of dopamine and DOPAC was reduced by 29 and 37%, respectively, (P < 0.05) and ouabain (500 and 1,000 muM) was no longer able to reduce the accumulation of either newly formed dopamine or DOPAC. In conclusion, the results presented here show that the formation of dopamine in kidney slices loaded with L-DOPA is partially dependent on extracellular sodium and partially sensitive to ouabain, suggesting the involvement of a cotransport system of sodium and L-DOPA into the tubular epithelial cell.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 9