Bioactive starch-based scaffolds and human adipose stem cells are a good combination for bone tissue engineering

• Type: Article in International Scientific Journal
• Date: 2012

Authors

Rodrigues, AI (Author) Other; Gomes, ME (Author) Other; Leonor, IB (Author) Other; Rui Reis (Author) Other

Journal

Title: Acta Biomaterialia

Vol. 8 No. 3

Pages: 3765-3776

ISSN: 1742-7061

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 52 Citations

Scopus - 60 Citations

Other information

Authenticus ID: P-002-4Z4

DOI: 10.1016/j.actbio.2012.05.025

Abstract (EN): Silicon is known to have an influence on calcium phosphate deposition and on the differentiation of bone precursor cells. This study explores the effect of the incorporation of silanol (Si-OH) groups into polymeric scaffolds on the osteogenic differentiation of human adipose stem cells (hASC) cultured under dynamic and static conditions. A blend of corn starch with polycaprolactone (30/70 wt.%, SPCL) was used to produce three-dimensional fibre meshes scaffolds by the wet-spinning technique, and a calcium silicate solution was used as a non-solvent to develop an in situ functionalization with Si-OH groups. In vitro assessment, using hASC, of functionalized and non-functionalized scaffolds was evaluated in either alpha-MEM or osteogenic medium under static and dynamic conditions (provided by a flow perfusion bioreactor). The functionalized materials, SPCL-Si, exhibit the capacity to sustain cell proliferation and induce their differentiation into the osteogenic lineage. The formation of mineralization nodules was observed in cells cultured on the SPCL-Si materials. Culturing under dynamic conditions using a flow perfusion bioreactor was shown to enhance the hASC proliferation and differentiation and a better distribution of cells within the material. The present work demonstrates the potential of these functionalized materials for future applications in bone tissue engineering. Additionally, these results highlight the simplicity, economic and reliable production process of those materials.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 12