Preliminary study on the adhesion and proliferation of human osteoblasts on starch-based scaffolds

• Type: Article in International Scientific Journal
• Date: 2002

Authors

Salgado, AJ (Author) Other; Gomes, ME (Author) Other; Chou, A (Author) Other; Coutinho, OP (Author) Other; Rui Reis (Author) Other; Hutmacher, DW (Author) Other

Journal

Title: Materials Science and Engineering C

Vol. 20

Pages: 27-33

ISSN: 0928-4931

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 97 Citations

Scopus - 109 Citations

Other information

Authenticus ID: P-000-PAD

DOI: 10.1016/s0928-4931(02)00009-7

Abstract (EN): Up to today, several techniques have been used to produce biodegradable porous scaffolds for tissue engineering. In this work, a new technique based on extrusion by using blowing agents in combination with a 50:50 (wt.%) blend of starch/cellulose acetate (SCA) was studied. The results show that by using this technique it was possible to obtain scaffolds with 70% of porosity and a fully interconnected network of pores, with sizes ranging from 200 to 500 pm. After their production, the mechanical properties of these scaffolds were tested, presenting a compressive modulus of 124.6 +/- 27.2 MPa and a compressive strength of 8.0 +/- 0.9 MPa. These values are within the best found in the literature and show that by using this technique, it is possible to produce scaffolds that, from a mechanical standpoint, may be suitable for bone tissue engineering. Cell culturing experiments showed that cells were viable and that there were no signs of cellular death after 3 weeks of culture. Finally, biochemical assays demonstrate that cells maintained the osteogenic phenotype throughout the experiment and deposition of mineralized extracellular matrix could be detected.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 7