Hybrid scaffolds for bone tissue engineering: Integration of composites and bioactive hydrogels loaded with hDPSCs

• Type: Article in International Scientific Journal
• Date: 2025

Authors

Sousa, AC (Author) Other; Alvites, R (Author) Other; Lopes, B (Author) Other; Sousa, P (Author) Other; Moreira, A (Author) Other; Coelho, A (Author) Other; Rêma, A (Author) Other; Biscaia, S (Author) Other; Cordeiro, R (Author) Other; Faria, F (Author) Other; da Silva, GF (Author) Other; Amorim, I (Author) Other; Santos, JD (Author) FEUP; Atayde, L (Author) Other; Alves, N (Author) Other; Domingos, M (Author) Other; Mauricio, AC (Author) ICBAS

Journal

The Journal is awaiting validation by the Administrative Services.

Title: BIOMATERIALS ADVANCES

Vol. 2

ISSN: 2772-9508

Indexing

ISI Web of Knowledge - 0 Citations

Scopus - 0 Citations

Other information

Authenticus ID: P-017-23Y

DOI: 10.1016/j.bioadv.2024.214042

Abstract (EN): Bone tissue regeneration remains a significant challenge in clinical settings due to the complexity of replicating the mechanical and biological properties of bone environment. This study addresses this challenge by proposing a hybrid scaffold designed to enhance both bioactivity and physical stability for bone tissue regeneration. This research is the fisrt to develop a rigid 3D structure composed of polycaprolactone (PCL) and hydroxyapatite nanoparticles (nHA) integrated with a bioink containing human dental pulp stem/stromal cells (hDPSCs), alginate, nHA and collagen (Col). The biofabricated constructs were extensively characterized through cytocompatibility tests, osteogenic differentiation assessment, and biocompatibility evaluation in a rat model. In vitro results demontrated that the hybrid scaffolds presented significantly higher cell viability after 168 h compared to the control group. Furthermore, the hybrid scaffolds showed increased osteogenic differentiation relative to other groups. In vivo evaluation indicated good biocompatibility, characterized by minimal inflammatory response and successful tissue integration. These findings highlight the scaffold's potential to support bone tissue regeneration by combining the mechanical strength of PCL and nHA with the biological activity of the alginate-nHA-Col and hDPSCs bioink. The current study provides a promising foundation for the development of biomaterials aimed at improving clinical outcomes in bone repair and regeneration, particulary for the treatment of critical-size bone defects, targeted drug administration, and three-dimensional models for bone tissue engineering.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 12