Go to:
Logótipo
Você está em: Start > Publications > View > Cationic Serine-Based Gemini Surfactant:Monoolein Aggregates as Viable and Efficacious Agents for DNA Complexation and Compaction: A Cytotoxicity and Physicochemical Assessment
Map of Premises
Principal
Publication

Cationic Serine-Based Gemini Surfactant:Monoolein Aggregates as Viable and Efficacious Agents for DNA Complexation and Compaction: A Cytotoxicity and Physicochemical Assessment

Title
Cationic Serine-Based Gemini Surfactant:Monoolein Aggregates as Viable and Efficacious Agents for DNA Complexation and Compaction: A Cytotoxicity and Physicochemical Assessment
Type
Article in International Scientific Journal
Year
2024-08-13
Authors
Sandra G. Silva
(Author)
FCUP
View Personal Page You do not have permissions to view the institutional email. Search for Participant Publications View Authenticus page Without ORCID
Isabel Oliveira
(Author)
FCUP
Andreia C. Gomes
(Author)
Other
The person does not belong to the institution. The person does not belong to the institution. The person does not belong to the institution. Without AUTHENTICUS Without ORCID
M. Elisabete C. D. Real Oliveira
(Author)
Other
The person does not belong to the institution. The person does not belong to the institution. The person does not belong to the institution. Without AUTHENTICUS Without ORCID
Journal
Vol. 15
Pages: 224-224
Publisher: MDPI
Indexing
Publicação em ISI Web of Knowledge ISI Web of Knowledge - 0 Citations
Publicação em Scopus Scopus - 0 Citations
Other information
Authenticus ID: P-013-HQ9
Abstract (EN): Cationic gemini surfactants have emerged as potential gene delivery agents as they can co-assemble with DNA due to a strong electrostatic association. Commonly, DNA complexation is enhanced by the inclusion of a helper lipid (HL), which also plays a key role in transfection efficiency. The formation of lipoplexes, used as non-viral vectors for transfection, through electrostatic and hydrophobic interactions is affected by various physicochemical parameters, such as cationic surfactant:HL molar ratio, (+/-) charge ratio, and the morphological structure of the lipoplexes. Herein, we investigated the DNA complexation ability of mixtures of serine-based gemini surfactants, (nSer)2N5, and monoolein (MO) as a helper lipid. The micelle-forming serine surfactants contain long lipophilic chains (12 to 18 C atoms) and a five CH2 spacer, both linked to the nitrogen atoms of the serine residues by amine linkages. The (nSer)2N5:MO aggregates are non-cytotoxic up to 35-90 mu M, depending on surfactant and surfactant/MO mixing ratio, and in general, higher MO content and longer surfactant chain length tend to promote higher cell viability. All systems efficaciously complex DNA, but the (18Ser)2N5:MO one clearly stands as the best-performing one. Incorporating MO into the serine surfactant system affects the morphology and size distribution of the formed mixed aggregates. In the low concentration regime, gemini-MO systems aggregate in the form of vesicles, while at high concentrations the formation of a lamellar liquid crystalline phase is observed. This suggests that lipoplexes might share a similar bilayer-based structure.
Language: English
Type (Professor's evaluation): Scientific
No. of pages: 13
Documents
We could not find any documents associated to the publication.
Related Publications

Of the same journal

Three-Dimensional Printing/Bioprinting and Cellular Therapies for Regenerative Medicine: Current Advances (2025)
Another Publication in an International Scientific Journal
Sousa, AC; Alvites, R; Lopes, B; Sousa, P; Moreira, A; Coelho, A; Santos, JD; Atayde, L; Alves, N; Mauricío, AC
The Use of Specialized Pro-Resolving Mediators in Biomaterial-Based Immunomodulation (2023)
Another Publication in an International Scientific Journal
Sousa, AB; Judite N Barbosa
The Role of Neutrophils in Biomaterial-Based Tissue Repair-Shifting Paradigms (2023)
Another Publication in an International Scientific Journal
Sousa, AB; Judite N Barbosa
Special Issue "Development of Biocomposites for Bone Tissue Engineering", Journal of Functional Biomaterials (2025)
Another Publication in an International Scientific Journal
Ferraz, MP; Nuno Alegrete
Special Issue "A New Era of Anti-infective Biomaterials for Biomedical Applications" (2022)
Another Publication in an International Scientific Journal
Ferraz, MP; Barros, J; Carvalho, A.

See all (12)

Recommend this page Top
Copyright 1996-2025 © Faculdade de Medicina Dentária da Universidade do Porto  I Terms and Conditions  I Acessibility  I Index A-Z
Page created on: 2025-07-09 at 06:12:22 | Privacy Policy | Personal Data Protection Policy | Whistleblowing | Electronic Yellow Book