The effect of hyaluronan-based delivery of stromal cell-derived factor-1 on the recruitment of MSCs in degenerating intervertebral discs

• Type: Article in International Scientific Journal
• Date: 2014

Authors

Pereira, CL (Author) Other; Goncalves, RM (Author) ICBAS; Peroglio, M (Author) Other; Pattappa, G (Author) Other; D'Este, M (Author) Other; Eglin, D (Author) Other; Barbosa, MA (Author) Other; Alini, M (Author) Other; Grad, S (Author) Other

Journal

Title: Biomaterials

Vol. 35

Pages: 8144-8153

ISSN: 0142-9612

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 68 Citations

Scopus - 83 Citations

Other information

Authenticus ID: P-009-NXP

DOI: 10.1016/j.biomaterials.2014.06.017

Abstract (EN): Intervertebral disc (IVD) degeneration is the leading cause of low back pain and disability in the active population. Transplantation of mesenchymal stem cells (MSCs) in a hydrogel carrier can induce regenerative effects in degenerated IVDs. Moreover, it was found that degenerative discs release chemo-attractants effective in MSC recruitment. Based on these findings, we hypothesized that an injectable hydrogel that can enhance the number of migrated MSCs in the IVD and provide a suitable matrix for their survival and differentiation would be ideal. The purpose of this study was to evaluate the potential of a thermoreversible hyaluronan-poly(N-isopropylacrylamide) (HAP) hydrogel as chemoattractant delivery system to recruit human MSCs in degenerative IVDs. The results demonstrate that HAP hydrogels containing stromal cell derived factor-1 (SDF-1) significantly increased the number of MSCs migrating into nucleotomized discs compared with discs treated with only HAP or SDF-1 in solution. HAP hydrogels releasing SDF-1 enhanced both the number of recruited cells and their migration distance in the IVD tissue. Furthermore, this phenomenon was dependent on MSC donor age. In conclusion, HAP SDF-1 is effective for the recruitment of stem cells in the IVD, thus opening new possibilities for the development of regenerative therapies based on endogenous cell migration.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 10