The impact of matrix age on intervertebral disc regeneration

• Type: Article in International Scientific Journal
• Date: 2022

Authors

Fiordalisi, MF (Author) Other; Ferreira, JR (Author) Other; Pinto, ML (Author) Other; Ribeiro Machado, C (Author) Other; Pinto, MT (Author) Other; Oliveira, MJ (Author) Other; Barbosa, MA (Author) Other; Goncalves, RM (Author) ICBAS; Caldeira, J (Author) Other

Journal

The Journal is awaiting validation by the Administrative Services.

Title: BIOMATERIALS ADVANCES

Vol. 143

ISSN: 2772-9508

Indexing

ISI Web of Knowledge - 3 Citations

Scopus - 4 Citations

Other information

Authenticus ID: P-00X-GAJ

DOI: 10.1016/j.bioadv.2022.213192

Abstract (EN): With the lack of effective treatments for low back pain, the use of extracellular matrix (ECM)-based biomaterials have emerged with undeniable promise for IVD regeneration. Decellularized scaffolds can recreate an ideal microenvironment inducing tissue remodeling and repair. In particular, fetal tissues have a superior regenerative capacity given their ECM composition. In line with this, we unraveled age-associated alterations of the nucleus pulposus (NP) matrisome. Thus, the aim of the present work was to evaluate the impact of ECM donor age on IVD de/regeneration.Accordingly, we optimized an SDS (0.1 %, 1 h)-based decellularization protocol that preserves ECM cues in bovine NPs from different ages. After repopulation with adult NP cells, younger matrices showed the highest repopulation efficiency. Most importantly, cells seeded on younger scaffolds produced healthy ECM proteins suggesting an increased capacity to restore a functional IVD microenvironment. In vivo, only fetal matrices decreased neovessel formation, showing an anti-angiogenic potential.Our findings demonstrate that ECM donor age has a strong influence on angiogenesis and ECM de novo syn-thesis, opening new avenues for novel therapeutic strategies for the IVD. Additionally, more appropriate 3D models to study age-associated IVD pathology were unveiled.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 16