Experimental Characterization of a Microfluidic Device Based on Passive Crossflow Filters for Blood Fractionation

• Type: Article in International Scientific Journal
• Date: 2022

Authors

Goncalves, IM (Author) Other; Castro, I (Author) Other; Barbosa, F (Author) Other; Faustino, V (Author) Other; Catarino, SO (Author) Other; Moita, A (Author) Other; Miranda, JM (Author) FEUP; Minas, G (Author) Other; Sousa, PC (Author) Other; Lima, R (Author) Other

Journal

The Journal is awaiting validation by the Administrative Services.

Title: PROCESSES

Vol. 26

Final page: 2698

ISSN: 2227-9717

Indexing

ISI Web of Knowledge - 0 Citations

Scopus - 3 Citations

Other information

Authenticus ID: P-00X-QMF

DOI: 10.3390/pr10122698

Abstract (EN): The separation of red blood cells (RBCs) from blood plasma and the analysis of individual RBCs are of great importance, as they provide valuable information regarding the health of their donor. Recent developments in microfluidics and microfabrication have contributed to the fabrication of microsystems with complex features to promote the separation and analysis of RBCs. In this work, the separation capacity of a multi-step crossflow microfluidic device was evaluated by using a blood analogue fluid made by Brij L4 micelles and human RBCs separated from whole blood, suspended in a solution with hematocrits (Ht) of 0.5 and 1%. All the samples collected at the outlets of the device were experimentally analyzed and compared. The absorbance spectrum was also measured for the prepared blood samples. The results indicate that the tested blood analogue fluid has exhibited a flow behavior similar to that of blood. In addition, the optical absorbance spectrophotometry revealed that it was possible to evaluate the separation efficiency of the microfluidic device, concluding that the concentration of cells was lower at the most lateral outside outlets of the microchannel due to the cumulative effect of the multiple cross-flow filters.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 12