Automatic flow procedure for the determination of glycerol in wine using enzymatic reaction and spectrophotometry

• Type: Article in International Scientific Journal
• Date: 2004

Authors

Elizabeth Nunes Fernandes (Author) Other; Mariele Nair de Campos Moura (Author) Other; José Luis F. Costa Lima (Author) FFUP; Boaventura F. Reis (Author) Other

Journal

Title: Mikrochimica Acta

Vol. 77 No. 2

Pages: 107-112

ISSN: 0026-3672

Publisher: Springer Nature

Indexing

ISI Web of Knowledge - 15 Citations

ISI Web of Science

Scopus - 16 Citations

Scientific classification

FOS: Medical and Health sciences > Other medical sciences

CORDIS: Health sciences

Other information

Authenticus ID: P-000-999

Resumo (PT): An automatic flow procedure for the determination of glycerol in wines by employing a flow system based on multicommutation and enzymatic reaction is described. Glycerol dehydrogenase was immobilized on aminopropyl glass beads and packed into a column that was coupled to the flow system. The NADH produced by the enzymatic reaction was monitored by spectrophotometry at 340 nm and its radiation absorption presented a relationship with glycerol concentration. The system manifold comprised a set of three-way solenoid valves controlled by a microcomputer, which was furnished with electronic interfaces and runs a software that was designed to carry out on-line sample dilution, reagent addition, and data acquisition. The procedure allows the determination of glycerol in wine samples without any prior pretreatment. The procedure presented as profitable features a linear response range between 2.0 and 10.0 g l−1 glycerol (R=0.998), a detection limit of 0.006 g l−1 glycerol, a relative standard deviation of 1.8% (n=14) for a typical wine sample presenting 5.3 g l−1 glycerol, a sampling throughput of 33 determinations per hour, and a NAD+ consumption of 0.8 mg per determination. The results were compared with those obtained using a reference method and no significant difference at 90% confidence level was observed. <br> <br> Keywords: Glycerol; Wine; On-line dilution; Multicommutation; Flow analysis; Glycerol dehydrogenase; Spectrophotometry <br> <a target="_blank" href="http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6W6H-4CBV1BN-1&_user=2460038&_coverDate=08%2F31%2F2004&_rdoc=5&_fmt=high&_orig=browse&_srch=doc-info(%23toc%236599%232004%23999229997%23507262%23FLA%23display%23Volume)&_cdi=6599&_sort=d&_docanchor=&_ct=19&_acct=C000057398&_version=1&_urlVersion=0&_userid=2460038&md5=99c97ae057bdb82bf70594adb9412291"> Texto integral</a> <br> <br>

Abstract (EN): An automatic flow procedure for the determination of glycerol in wines by employing a flow system based on multicommutation and enzymatic reaction is described. Glycerol dehydrogenase was immobilized on aminopropyl glass beads and packed into a column that was coupled to the flow system. The NADH produced by the enzymatic reaction was monitored by spectrophotometry at 340 nm and its radiation absorption presented a relationship with glycerol concentration. The system manifold comprised a set of three-way solenoid valves controlled by a microcomputer, which was furnished with electronic interfaces and runs a software that was designed to carry out on-line sample dilution, reagent addition, and data acquisition. The procedure allows the determination of glycerol in wine samples without any prior pretreatment. The procedure presented as profitable features a linear response range between 2.0 and 10.0 g l−1 glycerol (R=0.998), a detection limit of 0.006 g l−1 glycerol, a relative standard deviation of 1.8% (n=14) for a typical wine sample presenting 5.3 g l−1 glycerol, a sampling throughput of 33 determinations per hour, and a NAD+ consumption of 0.8 mg per determination. The results were compared with those obtained using a reference method and no significant difference at 90% confidence level was observed. <br> <br> Keywords: Glycerol; Wine; On-line dilution; Multicommutation; Flow analysis; Glycerol dehydrogenase; Spectrophotometry <br> <a target="_blank" href="http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6W6H-4CBV1BN-1&_user=2460038&_coverDate=08%2F31%2F2004&_rdoc=5&_fmt=high&_orig=browse&_srch=doc-info(%23toc%236599%232004%23999229997%23507262%23FLA%23display%23Volume)&_cdi=6599&_sort=d&_docanchor=&_ct=19&_acct=C000057398&_version=1&_urlVersion=0&_userid=2460038&md5=99c97ae057bdb82bf70594adb9412291"> Full text</a> <br> <br>

Language: Portuguese

Type (Professor's evaluation): Scientific