Physical–chemical parameters and validation of a colorimetric method for deoxycholic and ursodeoxycholic acids: kit reagent and optical sensor

• Type: Article in International Scientific Journal
• Date: 2011

Authors

Pabyton G. Cadena (Author) Other; Alberto N. Araújo (Author) FFUP; Maria C.B.S.M. Montenegro (Author) FFUP; Maria C.B. Pimentel (Author) Other; José L. Lima Filho (Author) Other; Valdinete L. Silva (Author) Other

Journal

Title: Chemistry and Physics of Lipids

Vol. 164 No. 2

Pages: 99-105

ISSN: 0009-3084

Publisher: Elsevier

Indexing

ISI Web of Science

Scopus

Pubmed / Medline

Scientific classification

FOS: Medical and Health sciences > Other medical sciences

CORDIS: Health sciences > Pharmacological sciences

Other information

Resumo (PT): The simple and low cost β-cyclodextrin (β-CD)–phenolphthalein (PHP) inclusion complex was used for both the study of physical–chemical parameters and validation of analytical procedures for deoxycholic acid (DCA) and ursodeoxycholic acid (UDCA) determinations in different formulations. The usefulness of this inclusion complex is proposed either in the form of kit reagent and as an original optical sensor for DCA and UDCA. The results showed that temperature had a negative effect on the equilibrium constant resulting in high negative values of enthalpy and positive values of entropy. The half-life values for DCA and UDCA measurements were 68.71 and 294.71 days, respectively. The method was validated showing limits of detection and quantification of 4.92 × 10−5 mol L−1 and 1.64 × 10−4 mol L−1 for DCA, 1.14 × 10−5 mol L−1 and 3.79 × 10−5 mol L−1 for UDCA, respectively. The developed optical sensor also showed response linearity, ease of implementation and potential application in fast screening tasks even out of the laboratory. <br> <br> Keywords: Deoxycholic acid; Ursodeoxycholic acid; β-Cyclodextrin; Inclusion complex; Phenolphthalein; Optical sensor <br> <a target="_blank" href="http://www.sciencedirect.com/science/article/pii/S0009308410003737 "> Texto integral</a> <br> <br>

Abstract (EN): The simple and low cost β-cyclodextrin (β-CD)–phenolphthalein (PHP) inclusion complex was used for both the study of physical–chemical parameters and validation of analytical procedures for deoxycholic acid (DCA) and ursodeoxycholic acid (UDCA) determinations in different formulations. The usefulness of this inclusion complex is proposed either in the form of kit reagent and as an original optical sensor for DCA and UDCA. The results showed that temperature had a negative effect on the equilibrium constant resulting in high negative values of enthalpy and positive values of entropy. The half-life values for DCA and UDCA measurements were 68.71 and 294.71 days, respectively. The method was validated showing limits of detection and quantification of 4.92 × 10−5 mol L−1 and 1.64 × 10−4 mol L−1 for DCA, 1.14 × 10−5 mol L−1 and 3.79 × 10−5 mol L−1 for UDCA, respectively. The developed optical sensor also showed response linearity, ease of implementation and potential application in fast screening tasks even out of the laboratory. <br> <br> Keywords: Deoxycholic acid; Ursodeoxycholic acid; β-Cyclodextrin; Inclusion complex; Phenolphthalein; Optical sensor <br> <a target="_blank" href="http://www.sciencedirect.com/science/article/pii/S0009308410003737 "> Full text</a> <br> <br>

Language: English

Type (Professor's evaluation): Scientific