Effect of anti-inflammatory drugs on splenocyte membrane fluidity

• Type: Article in International Scientific Journal
• Date: 2005

Authors

Ferreira, H (Author) Other; Lucio, M (Author) Other; Lima, JLFC (Author) FFUP; Cordeiro da Silva, A (Author) FFUP; Tavares, J (Author) FFUP; Reis, S (Author) FFUP

Journal

Title: Analytical Biochemistry

Vol. 339

Pages: 144-149

ISSN: 0003-2697

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 27 Citations

ISI Web of Science

Scopus - 30 Citations

Scientific classification

FOS: Natural sciences > Chemical sciences

CORDIS: Health sciences

Other information

Authenticus ID: P-000-3WY

DOI: 10.1016/j.ab.2004.12.023

Resumo (PT): In this study, fluorescence anisotropy measurements were performed using the fluorescent probe 1,6-diphenyl-1,3,5-hexatriene to investigate the effects on membrane fluidity resulting from the interaction between nonsteroidal anti-inflammatory drugs (NSAIDs)—indomethacin, diclofenac, piroxicam, tenoxicam, indoprofen, clonixin, and etodolac—and mouse splenocyte membranes. This study was performed in splenocyte membranes because most of the fluidity studies have been performed in membrane models; thus, clear correlations of the pharmacological action of drugs with molecular effects at the cellular membrane level were lacking. Besides providing a basis for studying the molecular mechanism of pharmacological action of NSAIDs, this research provides a data analysis of steady-state anisotropy measurements, taking into account that the probe itself strongly influences the data given that this problem is usually overlooked. Results show that the anti-inflammatory drugs indomethacin, diclofenac, piroxicam, and tenoxicam increase the membrane fluidity in a concentration-dependent manner. Their order of effectiveness reflected in their respective IC50 values (concentration of each NSAID required to increase the fluidizing effect ratio by 50%) is as follows: tenoxicam > piroxicam > indomethacin > clonixin. For the other drugs, the perturbation in membrane fluidity is not evident under these circumstances. <br> <br> Keywords: Nonsteroidal anti-inflammatory drugs; Splenocytes; 1,6-Diphenyl-1,3,5-hexatriene; Fluorescence anisotropy <br> <a target="_blank" href="http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6W9V-4F94G6D-2&_user=2460038&_coverDate=04%2F01%2F2005&_rdoc=21&_fmt=high&_orig=browse&_srch=doc-info(%23toc%236692%232005%23996609998%23580927%23FLA%23display%23Volume)&_cdi=6692&_sort=d&_docanchor=&_ct=29&_acct=C000057398&_version=1&_urlVersion=0&_userid=2460038&md5=eb421fb1acfc869a2a49983c8a5e4fd6"> Texto integral</a> <br> <br>

Abstract (EN): In this study, fluorescence anisotropy measurements were performed using the fluorescent probe 1,6-diphenyl-1,3,5-hexatriene to investigate the effects on membrane fluidity resulting from the interaction between nonsteroidal anti-inflammatory drugs (NSA-IDs)-indomethacin, diclofenac, piroxicam, tenoxicam, indoprofen, clonixin, and etodolac-and mouse splenocyte membranes. This study was performed in splenocyte membranes because most of the fluidity studies have been performed in membrane models; thus, clear correlations of the pharmacological action of drugs with molecular effects at the cellular membrane level were lacking. Besides providing a basis for studying the molecular mechanism of pharmacological action of NSAIDs, this research provides a data analysis of steady-state anisotropy measurements, taking into account that the probe itself strongly influences the data given that this problem is usually overlooked. Results show that the anti-inflammatory drugs indomethacin, diclofenac, piroxicam, and tenoxicam increase the membrane fluidity in a concentration-dependent manner. Their order of effectiveness reflected in their respective IC50 values (concentration of each NSAID required to increase the fluidizing effect ratio by 50%) is as follows: tenoxicam > piroxicam > indomethacin > clonixin. For the other drugs, the perturbation in membrane fluidity is not evident under these circumstances.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 6