Immune response by nasal delivery of hepatitis B surface antigen and codelivery of a CpG ODN in alginate coated chitosan nanoparticles

• Type: Article in International Scientific Journal
• Date: 2008

Authors

Olga Borges (Author) Other; Anabela Cordeiro da Silva (Author) FFUP; Joana Tavares (Author) FFUP; Nuno Santarem (Author) Other; Adriano de Sousa (Author) Other; Gerrit Borchard (Author) Other; Hans E Junginger (Author) Other

Journal

Title: European Journal of Pharmaceutics and Biopharmaceutics

Vol. 69 No. 2

Pages: 405-416

ISSN: 0939-6411

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 94 Citations

ISI Web of Science

Scopus - 122 Citations

Scientific classification

FOS: Medical and Health sciences > Basic medicine

CORDIS: Health sciences

Other information

Authenticus ID: P-003-YRX

DOI: 10.1016/j.ejpb.2008.01.019

Resumo (PT): Alginate coated chitosan nanoparticles were previously developed with the aim of protecting the antigen, adsorbed on the surface of those chitosan nanoparticles, from enzymatic degradation at mucosal surfaces. In this work, this new delivery system was loaded with the recombinant hepatitis B surface antigen (HBsAg) and applied to mice by the intranasal route. Adjuvant effect of the delivery system was studied by measuring anti-HBsAg IgG in serum, anti-HBsAg sIgA in faeces extracts or nasal and vaginal secretions and interferon-γ production in supernatants of the spleen cells. The mice were primed with 10 μg of the vaccine associated or not with nanoparticles and associated or not with 10 μg CpG oligodeoxynucleotide (ODN) followed by two sequential boosts at three week intervals. The association of HBsAg with the alginate coated chitosan nanoparticles, administered intranasally to the mice, gave rise to the humoral mucosal immune response. Humoral systemic immune response was not induced by the HBsAg loaded nanoparticles alone. The generation of Th1-biased antigen-specific systemic antibodies, however, was observed when HBsAg loaded nanoparticles were applied together with a second adjuvant, the immunopotentiator, CpG ODN. Moreover, all intranasally vaccinated groups showed higher interferon-γ production when compared to naïve mice. <br> <br> Keywords: Intranasal vaccination; Hepatitis B surface antigen; CpG oligodeoxynucleotide; Alginate coated chitosan nanoparticles; Vaccines <br> <a target="_blank" href="http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T6C-4RR1NPN-2&_user=2460038&_coverDate=06%2F30%2F2008&_rdoc=2&_fmt=high&_orig=browse&_srch=doc-info(%23toc%235027%232008%23999309997%23689777%23FLA%23display%23Volume)&_cdi=5027&_sort=d&_docanchor=&_ct=45&_acct=C000057398&_version=1&_urlVersion=0&_userid=2460038&md5=7587d60ad43530de4e3cdb6184e571b6"> Texto integral</a> <br> <br>

Abstract (EN): Alginate coated chitosan nanoparticles were previously developed with the aim of protecting the antigen, adsorbed on the surface of those chitosan nanoparticles, from enzymatic degradation at mucosal surfaces. In this work, this new delivery system was loaded with the recombinant hepatitis B surface antigen (HBsAg) and applied to mice by the intranasal route. Adjuvant effect of the delivery system was studied by measuring anti-HBsAg IgG in serum, anti-HBsAg sIgA in faeces extracts or nasal and vaginal secretions and interferon-gamma production in supernatants of the spleen cells. The mice were primed with 10 mu g of the vaccine associated or not with nanoparticles and associated or not with 10 mu g CpG oligodeoxynucleotide (ODN) followed by two sequential boosts at three week intervals. The association of HBsAg with the alginate coated chitosan nanoparticles, administered intranasally to the mice, gave rise to the humoral mucosal immune response. Humoral systemic immune response was not induced by the HBsAg loaded nanoparticles alone. The generation of Th1-biased antigen-specific systemic antibodies, however, was observed when HBsAg loaded nanoparticles were applied together with a second adjuvant, the immumopotentiator, CpG ODN. Moreover, all intranasally vaccinated groups showed higher interferon-gamma production when compared to naive mice.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 12