Method development and validation for isoflavones quantification in coffee

• Type: Article in International Scientific Journal
• Date: 2010

Authors

R. C. Alves (Author) FFUP; I.M.C. Almeida (Author) Other; S Casal (Author) FFUP; M.B.P.P. Oliveira (Author) FFUP

Journal

Title: Food Chemistry

Vol. 122 No. 3

Pages: 914-919

ISSN: 0308-8146

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 17 Citations

ISI Web of Science

Scopus - 18 Citations

Scientific classification

FOS: Medical and Health sciences > Other medical sciences

CORDIS: Health sciences

Other information

Authenticus ID: P-003-2CV

Resumo (PT): This paper reports the development and validation of an analytical method to quantify three isoflavones (daidzein, genistein and formononetin) in coffee beans and coffee brews, by HPLC with diode-array detection. The aglycones were released by methanolic acid hydrolysis in the presence of the antioxidant BHT and the internal standard 2′-methoxyflavone. The method showed high correlation coefficients (r > 0.999) for standards subjected to the entire procedure. For samples, good intra- and inter-day precisions (<8%), and accuracies (recoveries of 95 ± 1% for ground coffee and 92 ± 2% for coffee brew) were achieved. Detection and quantification limits ranged from 5 to 8 ng/mL and from 14 and 25 ng/mL, respectively. The proposed method proved to be sensitive, precise and accurate. <br> <br> Keywords: Isoflavones; Daidzein; Genistein; Formononetin; coffee; Hydrolysis; HPLC; Diode-array <br> <a target="_blank" href="http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T6R-4YMPXCY-4&_user=2460038&_coverDate=10%2F01%2F2010&_rdoc=68&_fmt=high&_orig=browse&_origin=browse&_zone=rslt_list_item&_srch=doc-info(%23toc%235037%232010%23998779996%231955707%23FLA%23display%23Volume)&_cdi=5037&_sort=d&_docanchor=&_ct=72&_acct=C000057398&_version=1&_urlVersion=0&_userid=2460038&md5=2788a1e3815617d47a035dae3f9c2dc3&searchtype=a"> Texto integral</a> <br> <br>

Abstract (EN): This paper reports the development and validation of an analytical method to quantify three isoflavones (daidzein, genistein and formononetin) in coffee beans and coffee brews, by HPLC with diode-array detection. The aglycones were released by methanolic acid hydrolysis in the presence of the antioxidant BHT and the internal standard 2′-methoxyflavone. The method showed high correlation coefficients (r > 0.999) for standards subjected to the entire procedure. For samples, good intra- and inter-day precisions (<8%), and accuracies (recoveries of 95 ± 1% for ground coffee and 92 ± 2% for coffee brew) were achieved. Detection and quantification limits ranged from 5 to 8 ng/mL and from 14 and 25 ng/mL, respectively. The proposed method proved to be sensitive, precise and accurate. <br> <br> Keywords: Isoflavones; Daidzein; Genistein; Formononetin; coffee; Hydrolysis; HPLC; Diode-array <br> <a target="_blank" href="http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T6R-4YMPXCY-4&_user=2460038&_coverDate=10%2F01%2F2010&_rdoc=68&_fmt=high&_orig=browse&_origin=browse&_zone=rslt_list_item&_srch=doc-info(%23toc%235037%232010%23998779996%231955707%23FLA%23display%23Volume)&_cdi=5037&_sort=d&_docanchor=&_ct=72&_acct=C000057398&_version=1&_urlVersion=0&_userid=2460038&md5=2788a1e3815617d47a035dae3f9c2dc3&searchtype=a"> Full text</a> <br> <br>

Language: English

Type (Professor's evaluation): Scientific