Optical immunosensor panel using quantum dot-antibody conjugates for highly sensitive detection of carbohydrate antigen 19-9 (CA19-9)

• Type: Article in International Scientific Journal
• Date: 2025

Authors

Oliveira, D (Author) Other; Oliveira, P (Author) Other; Xu, AA (Author) Other; Rodrigues, E (Author) Other; Guerreiro, SG (Author) Other; Castro, RC (Author) Other; Ribeiro, DSM (Author) Other; Joao L M Santos (Author) FFUP; Piloto, AML (Author) Other

Journal

Title: Analytica Chimica Acta

Vol. 1333

ISSN: 0003-2670

Publisher: Elsevier

Indexing

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Other information

Authenticus ID: P-017-E8F

DOI: 10.1016/j.aca.2024.343399

Abstract (EN): This study introduces a fluorescent immunosensor colour panel with antibody-conjugated quantum dots for detecting CA19-9 in human serum. The immunosensors presented decreasing fluorescence with increasing CA19-9 concentrations, with a linear detection range from 0.01 to 501.87 U mL(-1) and detection limits of 1.66 x 10(-4), 2.71 x 10(-4), and 5.45 x 10(-4) U mL(-1) for the green-, orange-, and red-emitting conjugates in human serum. These limits are well below the 37 U mL(-1) cutoff for early pancreatic cancer risk. The immunosensor panel is simple, sensitive, and specific, offering a visual colour readout for rapid CA19-9 detection, making it ideal for point-of-care applications. The results highlight the potential of the developed conjugates as a sensitive fluorescence colour panel for detecting CA19-9 across a wide range in human serum, both below and above the pancreatic cancer cutoff. With just 200 mu L of sample and a single pre-filtration step, the assay delivers results in under 15 min, offering a cost-effective and efficient platform for point-of-care use.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 12