Dissecting the Mechanical Control of Mitotic Entry Using a Cell Confinement Setup

• Type: Article in International Scientific Journal
• Date: 2024

Authors

Dantas, M (Author) Other; Vareiro, D (Author) Other; J Ferreira (Author) FMUP

Journal

The Journal is awaiting validation by the Administrative Services.

Title: BIO-PROTOCOL

Vol. 14

ISSN: 2331-8325

Indexing

ISI Web of Knowledge - 0 Citations

Scopus - 0 Citations

Other information

Authenticus ID: P-010-94H

DOI: 10.21769/bioprotoc.4959

Abstract (EN): Proliferating cells need to cope with extensive cytoskeletal and nuclear remodeling as they prepare to divide. These events are tightly regulated by the nuclear translocation of the cyclin B1-CDK1 complex, that is partly dependent on nuclear tension. Standard experimental approaches do not allow the manipulation of forces acting on cells in a timeresolved manner. Here, we describe a protocol that enables dynamic mechanical manipulation of single cells with high spatial and temporal resolution and its application in the context of cell division. In addition, we also outline a method for the manipulation of substrate stiffness using polyacrylamide hydrogels. Finally, we describe a static cell confinement setup, which can be used to study the impact of prolonged mechanical stimulation in populations of cells.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 12