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Nanobody-based immunosensor for the detection of <i>H. pylori</i> in saliva

Title
Nanobody-based immunosensor for the detection of <i>H. pylori</i> in saliva
Type
Article in International Scientific Journal
Year
2024
Authors
Ahmad, MIA
(Author)
Other
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Amorim, CG
(Author)
FFUP
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Abu Qatouseh, LF
(Author)
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Montenegro, MCBSM
(Author)
FFUP
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Journal
Vol. 260
ISSN: 0956-5663
Publisher: Elsevier
Other information
Authenticus ID: P-010-GHE
Abstract (EN): Helicobacter pylori (H. pylori) infection is highly prevalent worldwide, affecting more than 43% of world population. The infection can be transmitted through different routes, like oral-oral, fecal-oral, and gastric-oral. Electrochemical sensors play a crucial role in the early detection of various substances, including biomolecules. In this study, the development of nanobody (Nb)-based immunosensor for the detection of H. pylori antigens in saliva samples was investigated. The D2_Nb was isolated and characterized using Western blot and ELISA and employed in the fabrication of the immunosensor. The sensor was prepared using gold screen-printed electrodes, with the immobilization of Nb achieved through chemical linkage using cysteamine-glutaraldehyde. The surface of the electrode was characterized using EIS, FTIR and SEM. Initially, the Nb-based immunosensor's performance was evaluated through cyclic voltammetry (CV), differential pulse voltammetry (DPV), and square wave voltammetry (SWV). The sensor exhibited excellent linearity with an R-2 value of 0.96. However, further assessment with the DPV technique revealed both a low limit of detection (5.9 ng/mL, <1 cfu/mL) and high selectivity when exposed to a mixture of similar antigens. Moreover, the immunosensor demonstrated robust recovery rates (96.2%-103.4%) when spiked into artificial saliva and maintained its functionality when stored at room temperature for 24 days.
Language: English
Type (Professor's evaluation): Scientific
No. of pages: 12
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