The characteristic time of glucose diffusion measured for muscle tissue at optical clearing

• Type: Article in International Scientific Journal
• Date: 2013

Authors

Luís Oliveira (Author) FEUP; Maria Inês Barbosa De Carvalho (Author) FEUP; Elisabete Nogueira (Author) Other; Valery V. Tuchin (Author) Other

Journal

Title: Laser Physics

Vol. 23

Final page: 075606

ISSN: 1054-660X

Publisher: Institute of Physics Publishing

Indexing

ISI Web of Knowledge - 24 Citations

ISI Web of Science

Scopus - 29 Citations

Scientific classification

FOS: Natural sciences > Physical sciences

Other information

Authenticus ID: P-004-ZTY

DOI: 10.1088/1054-660x/23/7/075606

Abstract (EN): The study of agent diffusion in biological tissues is very important to understand and characterize the optical clearing effects and mechanisms involved: tissue dehydration and refractive index matching. From measurements made to study the optical clearing, it is obvious that light scattering is reduced and that the optical properties of the tissue are controlled in the process. On the other hand, optical measurements do not allow direct determination of the diffusion properties of the agent in the tissue and some calculations are necessary to estimate those properties. This fact is imposed by the occurrence of two fluxes at optical clearing: water typically directed out of and agent directed into the tissue. When the water content in the immersion solution is approximately the same as the free water content of the tissue, a balance is established for water and the agent flux dominates. To prove this concept experimentally, we have measured the collimated transmittance of skeletal muscle samples under treatment with aqueous solutions containing different concentrations of glucose. After estimating the mean diffusion time values for each of the treatments we have represented those values as a function of glucose concentration in solution. Such a representation presents a maximum diffusion time for a water content in solution equal to the tissue free water content. Such a maximum represents the real diffusion time of glucose in the muscle and with this value we could calculate the corresponding diffusion coefficient.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 6