Antimicrobial and antibiofilm effect of phytochemicals as a potential treatment against healthcare-associated infections

• Type: Summary of Presentation in a National Conference
• Date: 2023-05-12

Authors

Anabela Borges (Author) FEUP; Mariana Sousa (Author) FEUP; Lília S. Teixeira (Author) FEUP; Ana C. Afonso (Author) Other; Maria José Saavedra (Author) Other; Lúcia C. Simões (Author) Other; Simões M (Author) FEUP

Conference proceedings National

Title: IJUP: book of abstracts of the 16th U.PORTO Young Researchers Meeting| IJUP: Livro de Resumos do 16.º Encontro de Jovens Investigadores da U.PORTO

Pages: 367-368

IJUP: 16th U.PORTO Young Researchers Meeting| IJUP: 16.º Encontro de Jovens Investigadores da U.PORTO

Reitoria da Universidade do Porto, 10 a 12 Maio de 2023

Scientific classification

CORDIS: Natural sciences > Biological sciences

FOS: Medical and Health sciences > Health sciences

Associated Projects

LEPABE - Financiamento Plurianual 2020-2023 da Unidade de I&D LEPABE - Laboratório de Engenharia de Processos, Ambiente, Biotecnologia e Energia

LEPABE - Projeto Estratégico do LEPABE - Laboratório de Engenharia de Processos, Ambiente, Biotecnologia e Energia

ALiCE - ALiCE - Laboratório Associado em Engenharia Química

GermIrrad - Sistema automatizado de irradiação germicida

Other information

Abstract (EN): The treatment of bacterial infections has been troubled by the increased resistance to antibiotics [1, 2]. Thus, it is crucial to discover novel and effective therapies to control and eradicate planktonic and sessile bacterial cells [3]. Phytochemicals have demonstrated broad-spectrum and effective antibacterial effects as well as antibiotic resistance-modifying activity [4]. In this study, perillyl alcohol and hydrocinnamic acid were characterized for their antimicrobial and antibiofilm action against Escherichia coli CECT 434. Furthermore, dual and triple combinations of these phytochemicals with chloramphenicol and amoxicillin were investigated for the first time. Perillyl alcohol had a minimum inhibitory concentration (MIC) of 256 μg/mL and a minimum bactericidal concentration (MBC) of 512 μg/mL. Hydrocinnamic acid had a MIC of 2048 μg/mL and an MBC > 2048 μg/mL. Checkerboard demonstrated synergism or additive effects for chloramphenicol/perillyl alcohol, chloramphenicol/hydrocinnamic acid, and amoxicillin/hydrocinnamic acid. The analysis with Combenefit showed synergism for various concentrations of amoxicillin with each phytochemical and mainly at low concentrations of chloramphenicol. Regarding the biofilms, both phytochemicals provided a total elimination of colony-forming units (CFU), for 5×MIC and 10×MIC. The highest percentages of metabolic inactivation (88.5% ± 0.8% for 10×MIC) and biomass reduction (61.7% ± 1.6% for 10×MIC) were obtained for E. coli treated with amoxicillin. All combinations resulted in high efficacy concerning metabolic inactivation and revealed moderate efficacy in terms of biomass reduction. Considering the culturability of sessile cells, synergism was determined for 20.0% of combinations, additivity for 60.0%, and indifference for 20.0%. The results of this study highlighted the potential of combinatorial therapies for microbial and biofilm control, where phytochemicals play an important role as resistance-modifying agents.

Language: English

Type (Professor's evaluation): Scientific