Antimicrobial cyclodextrin-assisted electrospun fibers loaded with carvacrol, citronellol and cinnamic acid for wound healing

• Type: Article in International Scientific Journal
• Date: 2024

Authors

Gonzalez-Prada, I (Author) Other; Anabela Borges (Author) FEUP; Santos-Torres, B (Author) Other; Magariños, B (Author) Other; Simoes, M (Author) Other; Concheiro, A (Author) Other; Alvarez-Lorenzo, C (Author) Other; Simões M (Author) FEUP

Journal

Title: International Journal of Biological Macromolecules

Vol. 277

Pages: 1-20

ISSN: 0141-8130

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 0 Citations

Scopus - 0 Citations

Associated Projects

LEPABE - Financiamento Plurianual 2020-2023 da Unidade de I&D LEPABE - Laboratório de Engenharia de Processos, Ambiente, Biotecnologia e Energia

LEPABE - Projeto Estratégico do LEPABE - Laboratório de Engenharia de Processos, Ambiente, Biotecnologia e Energia

ALiCE - ALiCE - Laboratório Associado em Engenharia Química

InnovAntiBiofilm - Boosting Sustainable Innovation in Developing New Antibiotic Adjuvants to Control Biofilm Resistance

Other information

Authenticus ID: P-016-SR7

DOI: 10.1016/j.ijbiomac.2024.134154

Abstract (EN): This work aimed to explore an alternative to the use of antibiotics for prevention and treatment of wounds infection caused by two common bacterial pathogens Staphylococcus aureus and Pseudomonas aeruginosa. For this purpose, three different essential oil components (EOCs), namely carvacrol, citronellol and cinnamic acid, were loaded into electrospun fibers of poly-epsilon-caprolactone (PCL) aided by alpha-cyclodextrin (alpha CD) and hydroxypropyl-beta-cyclodextrin (HP beta CD). Electrospun-fibers prepared with each EOC and their mixtures were screened for antimicrobial capability and characterized regarding morphological, mechanical, thermal, surface polarity, antibiofilm and antioxidant properties. alpha CD formed poly(pseudo)rotaxanes with PCL and weakly interacted with EOCs, while HP beta CD facilitated EOC encapsulation and formation of homogeneous fibers (500-1000 nm diameter) without beads. PCL/HP beta CD fibers with high concentration of EOCs (mainly carvacrol and cinnamic acid) showed strong antibiofilm (>3 log CFU reduction) and antioxidant activity (10-50% DPPH scavenging effects). Different performances were recorded for the EOCs and their mixtures; cinnamic acid migrated to fiber surface and was released faster. Fibers biocompatibility was verified using hemolysis tests and in ovo tissue integration and angiogenesis assays. Overall, HP beta CD facilitates complete release of EOCs from the fibers to the aqueous medium, being an environment-friendly and cost-effective strategy for the treatment of infected wounds.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 20