Immunomodulatory effect of different dietary ARA/EPA/DHA ratios in gilthead sea bream (<i>Sparus aurata</i>) juveniles after infection with <i>Photobacterium damselae</i> subsp. <i>piscicida</i>

• Type: Article in International Scientific Journal
• Date: 2022

Authors

Magalhaes, R (Author) Other; Guardiola, FA (Author) Other; Guerreiro, I (Author) Other; Fontinha, F (Author) Other; Moutinho, S (Author) Other; Serra, CR (Author) Other; Olsen, RE (Author) Other; Peres, H (Author) FCUP; Oliva Teles, A (Author) FCUP

Journal

Title: Aquaculture Research

Vol. 53

Pages: 6007-6019

ISSN: 1355-557X

Publisher: Wiley-Blackwell

Indexing

ISI Web of Knowledge - 2 Citations

Scopus

Other information

Authenticus ID: P-00X-5GJ

DOI: 10.1111/are.16070

Abstract (EN): The immunomodulatory effect of different dietary arachidonic acid (ARA), eicosapentaenoic acid (EPA), and docosahexaenoic acid (DHA) ratios was evaluated in gilthead sea bream juveniles after intraperitoneal injection (i.p.) with formalin-killed Photobacterium damselae subsp. piscicida (Phdp). Fish were fed with four isoproteic (47% crude protein) and isolipidic (18% crude lipids) diets with the following long-chain polyunsaturated fatty acids (LC-PUFA) ratios: Diet A (2.0% ARA: 0.2% EPA: 0.1% DHA); Diet B (1.0% ARA: 0.4% EPA: 0.4% DHA); Diet C (0% ARA: 0.6% EPA: 0.6% DHA); Diet D (0% ARA: 0.3% EPA: 1.5% DHA). After being fed for 56 days with the experimental diets, fish from each dietary treatment were randomly selected and i.p. injected with 0.2 ml of killed Phdp (1 x 10(8) CFU ml(-1)) or phosphate-buffered saline (control group). Four and twenty-four hours (i.e., twice) after the i.p., the haematological profile, plasma humoral innate immune parameters, and the intestine expression of immune-related genes were assessed. Results showed that fish fed diet D (high DHA content) had increased complement activity and higher intestinal expression of cyclooxygenase 2 (cox(2)) and interleukin 1 beta (il-1 beta) at 4 and 24 hours after i.p., as well as increased intestinal tumour necrosis factor alpha (tnf alpha) expression at 4 hours. On the other hand, fish fed with diet A (high ARA content) showed higher plasma bactericidal activity against Phdp than fish fed with diet D. Overall, the results of this study suggest a positive modulatory effect of DHA in response to the pathogenic agent.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 13