NMR and EPR study of the interaction of tris(3-hydroxy-4-pyridinonato) Ga (III) complexes with liposomes that mimic plant membranes

• Type: Article in International Scientific Journal
• Date: 2023

Authors

Vinhas, S (Author) Other; Ivanova, G (Author) Other; de Castro, B (Author) Other; Maria Rangel (Author) ICBAS

Journal

Title: Biophysical Chemistry

Vol. 298

ISSN: 0301-4622

Publisher: Elsevier

Indexing

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Other information

Authenticus ID: P-00Y-E44

DOI: 10.1016/j.bpc.2023.107021

Abstract (EN): We performed an NMR and EPR study of the interaction of four [Ga(3,4-HPO)3] chelates with liposomes derived from a soybean extract (SEL) and simpler formulations using POPC (100%) and POPE:POPC (50%). Parent [Fe (3,4-HPO)3] chelates are eligible to prevent Iron Deficiency Chlorosis and we took advantage of the likenesses of the ions Fe (III) and Ga (III), and the fact their metal ion complexes are isostructural, to perform a combined NMR and EPR study to get information about the permeation properties of the complexes.The results demonstrate the presence of liposomes loaded with Ga-chelates and that the distribution of complexes alongside the bilayer is dependent on their structure. Two compounds, [Ga(mpp)3] and [Ga(etpp)3], have a higher affinity for the polar region of the liposome bilayer thus suggesting that their structure facilitates their permanence at the root-rhizosphere interface. Chelates [Ga(dmpp)3] and [Ga(mrb13)3] interact with all types of protons of the lipid bilayer thus implying that they travel all along the bilayer structure indicating their higher permeation properties through soybean membranes.The results obtained for compound, [Ga(mrb13)3], which has been included in this work but was not yet tested in plant supplementation experiments, encourage its testing in in vivo plant studies once this study revealed that it interacts strongly with the model membranes. If the results of the future experiments in plants are positive and consistent with the present membrane-interaction studies the latter could constitute a good screening test for future compounds thus saving reagents and time.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 11