Impact of umbilical cord mesenchymal stromal/stem cell secretome and cord blood serum in prostate cancer progression

• Type: Article in International Scientific Journal
• Date: 2023

Authors

Sousa, A (Author) Other; Coelho, P (Author) Other; Leite, F (Author) Other; Teixeira, C (Author) Other; Rocha, AC (Author) Other; Santos, I (Author) Other; Baylina, P (Author) Other; Fernandes, R (Author) Other; soares, r (Author) FMUP; Costa, R (Author) Other; Gomes, A (Author) Other

Journal

The Journal is awaiting validation by the Administrative Services.

Title: HUMAN CELL

Vol. 36

Pages: 1160-1172

ISSN: 0914-7470

Indexing

ISI Web of Knowledge - 2 Citations

Scopus - 8 Citations

Other information

Authenticus ID: P-00Y-0AR

DOI: 10.1007/s13577-023-00880-z

Abstract (EN): Prostate cancer (PCa) is the second most common malignancy in men, and the fifth leading cause of death worldwide. Mesenchymal stromal/stem cells (MSC) have been identified in PCa, although contradictory effects in malignant transformation and tumor progression have been described. Since umbilical cord (UC) MSC and cord blood serum (CBS) are rich in numerous growth and anti-inflammatory factors, UC-MSC secretome and CBS are able to modulate tumor cell proliferation and survival as well as immunity and angiogenesis. In the present study, we address this relationship and investigate the influence of UC-MSC secretome and CBS on two human PCa cell lines (PC3 and LNCaP) and a normal epithelial prostate cell line (HPEpiC). Our results disclosed that upon exposure to UC-MSC-conditioned medium or CBS, both PC3 and LNCaP cells exhibited reduced viability, proliferation, and motility while non-malignant epithelial prostate cells were unaffected. These findings were corroborated by expression analysis of AKT/PI3K signaling pathway, p53 and interleukin genes. UC-MSC and CBS factors decreased the expression of growth-stimulating AKT and PI3K effectors and simultaneously up-regulated the expression of tumor-suppressor p53. Moreover, a more anti-inflammatory expression profile was found in both malignant PCa cell lines. Altogether, these results shed light into possible mechanisms by which UC-MSC and CBS reduce PCa progression, further reinforcing their potential use as novel therapeutic agents in PCa. [GRAPHICS] .

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 13