Combined RxFISH/G-banding allows refined karyotyping of solid tumors

• Type: Article in International Scientific Journal
• Date: 1999

Authors

Micci, F (Author) Other; Manuel R Teixeira (Author) Other; Dietrich, CU (Author) Other; Saeter, G (Author) Other; Bjerkehagen, B (Author) Other; Heim, S (Author) Other

Journal

Title: Human Genetics

Vol. 104

Pages: 370-375

ISSN: 0340-6717

Publisher: Springer Nature

Indexing

ISI Web of Knowledge - 20 Citations

Scopus - 22 Citations

Other information

Authenticus ID: P-008-Q9T

DOI: 10.1007/s004390050970

Abstract (EN): Chromosome banding analysis of solid tumors often yields incomplete karyotypes because of the complex rearrangements encountered. The addition of fluorescence in situ hybridization (FISH) methods has helped improve the accuracy of solid tumor cytogenetics, but the absence of screening qualities from standard FISH approaches has proved a severe limitation. We describe the cytogenetic analysis of ten solid tumors using G-banding followed by cross-species color banding (RxFISH), a FISH-based screening technique giving a chromosome-specific banding pattern based on the genomic homologies between humans and gibbons. The addition of RxFISH analysis in all cases led to the identification of previously unidentified intra- as well as interchromosomal rearrangements, thus giving a much more certain and detailed karyotype. In two gastric stromal sarcomas, a tumor type for which no cytogenetic data were hitherto available, numerical chromosomal aberrations dominated, but one of the tumors also carried an unbalanced 7;17-translocation with the same breakpoint in chromosome 17 as that seen in endometrial stromal sarcomas.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 6