Turn-on, photostable, nontoxic and specific, iron(II) sensor

• Type: Article in International Scientific Journal
• Date: 2022

Authors

Goncalves, HMR (Author) Other; Tavares, IS (Author) Other; Neves, SAF (Author) FEUP; Fontes, R (Author) Other; Duarte, AJ (Author) Other

Journal

Title: Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy

Vol. 265

ISSN: 1386-1425

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 0 Citations

Scopus - 4 Citations

Other information

Authenticus ID: P-00V-FT1

DOI: 10.1016/j.saa.2021.120380

Abstract (EN): The pressing need to develop a specific analytical sensor that can identify and quantify Fe(II) without a cytotoxic response was the major motivation drive in this work. The turn-on fluorescent sensor here described can successfully detect Fe(II) and discriminate this ion from other analytes that commonly act as interferents in biological media. Moreover, this reduced fluoresceinamine-based sensor has a high photostability and high dissociation constant, which is an indication that the complex obtained between reduced fluoresceinamine (RFL) and Fe(II) is highly stable. This fluorescence-based sensor has a binding mechanism of 1:1 and a positive cooperativity was found between analyte and sensor. The detection, quantification and sensitivity parameters of the sensor were determined: 21.6 +/- 0.1 lM; 65.6 +/- 0.1 lM and 48 +/- 3 (x10(7)) lM, respectively. To evaluate a possible cytotoxicity effect an erythrocyte assay was performed and the obtained data were evaluated considering CdTe Quantum Dots (QDs) passivated with mercaptoacetic acid has experimental control. According to the resulting data RFL is not cytotoxic even when used in high concentrations, 660 mM. On the other hand QDs are quite different. Indeed it was proven that these heavy metal-based nanoparticles are responsible for 40% erytrocytes hemolysis in concentrations of 600 mM.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 9