Aeoliella straminimaris sp. nov., a novel member of the phylum Planctomycetota with an unusual filamentous structure

• Type: Article in International Scientific Journal
• Date: 2023

Authors

Vitorino, IR (Author) Other; Lobo-da-Cunha, A (Author) Other; Vitor Vasconcelos (Author) FCUP; lage, om (Author) FCUP

Journal

Title: International Journal of Systematic and Evolutionary Microbiology

Vol. 73

ISSN: 1466-5026

Publisher: Microbiology Society

Indexing

ISI Web of Knowledge - 0 Citations

Scopus

Other information

Authenticus ID: P-00Y-AQC

DOI: 10.1099/ijsem.0.005850

Abstract (EN): Organisms with distinctive biological features and cellular organization constitute the bacterial phylum Planctomycetota. In this study, we formally describe a novel isolate, strain ICT_H6.2(T), isolated from sediment samples collected in the brackish environment of the Tagus River estuary (Portugal) using an iChip- based culturing technique. The 16S rRNA gene analysis placed this strain into the phylum Planctomycetota and family Lacipirellulaceae, with a similarity value of 98.0 % to its closest relative Aeoliella mucimassa Pan181(T), the currently only known member of the genus. Strain ICT_H6.2(T) has a genome size of 7.8 Mbp and a DNA G+C content of 59.6 mol %. Strain ICT_H6.2(T) is heterotrophic, aerobic and capable of microaerobic growth. This strain grows from 10 to 37 degrees C and from pH 6.5 to 10.0, requires salt to grow, and can tolerate up to 4 % (w/v) NaCl. Diverse nitrogen and carbon sources are utilized for growth. Morphologically, strain ICT_H6.2(T) is white to beige pigmented, spherical to ovoid in shape and around 1.4x1.1 mu m in size. The strain clusters mainly in aggregates and younger cells show motility. Ultrastructural studies showed a cell plan with cytoplasmatic membrane invaginations and unusual filamentous structures with hexagonal organization in transversal section. Morphological, physiological and genomic comparison between strain ICT_H6.2(T) and its closest relatives strongly suggests it represents a novel species within the genus Aeoliella, for which we propose the name Aeoliella straminimaris sp. nov., represented by strain ICT_H6.2(T) as the type strain (=CECT 30574(T)=DSM 114064(T)).

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 13