Application of a dot blot hybridization assay for genotyping Streptococcus uberis from Brazilian dairy herds

• Type: Article in International Scientific Journal
• Date: 2021

Authors

Alves, BG (Author) Other; Martins, L (Author) Other; Ribeiro, JN (Author) Other; Fernando Tavares (Author) FCUP; dos Santos, MV (Author) Other

Journal

Title: Journal of Dairy Science

Vol. 104

Pages: 3418-3426

ISSN: 0022-0302

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 0 Citations

Scopus - 0 Citations

Other information

Authenticus ID: P-00T-8XC

DOI: 10.3168/jds.2020-18782

Abstract (EN): Streptococcus uberis is a major cause of environmental mastitis in many regions, and it is associated with clinical and subclinical infections. Although the main source of infection is the environment, reports of strains with a contagious profile have been described. Dot blot hybridization analysis allows the rapid identification of S. uberis population structures within and between herds, and it helps to identify strain diversity as well as possible clonal lineages that directly affect the control of bovine mastitis caused by this pathogen. The aim of this study was to evaluate the diversity of S. uberis isolates obtained from clinical (n = 22) and subclinical (n = 22) cases of mastitis in dairy herds (n = 13) in Brazil over a period of 12 mo. We submitted 44 S. uberis isolates to dot blot hybridization followed by automatic data analysis. We identified 8 different hybridization patterns using genetic markers associated with virulence factors and taxonomy, indicating diversity of S. uberis within the population and suggesting environmental transmission. However, the evidence of identical dot blot patterns in different mammary quarters from the same animal also suggested local contagious transmission. Of the virulence genes evaluated, we found a high prevalence of the genes sua, pauA, and gapC, highlighting the importance of these virulence factors for the adhesion, invasion, and multiplication of S. uberis in subclinical and clinical intramammary infections.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 9