ICEs Are the Main Reservoirs of the Ciprofloxacin-ModifyingcrpPGene inPseudomonas aeruginosa

• Type: Article in International Scientific Journal
• Date: 2020

Authors

Botelho, J (Author) Other; Grosso, F (Author) Other; Luisa Peixe (Author) FFUP

Journal

Title: Genes

Vol. 11

ISSN: 2073-4425

Publisher: MDPI

Indexing

ISI Web of Knowledge - 0 Citations

Scopus - 4 Citations

Other information

Authenticus ID: P-00S-K25

DOI: 10.3390/genes11080889

Abstract (EN): The ciprofloxacin-modifyingcrpPgene was recently identified in a plasmid isolated from aPseudomonas aeruginosaclinical isolate. Homologues of this gene were also identified inEscherichia coli,Klebsiella pneumoniaeandAcinetobacter baumannii. We set out to explore the mobile elements involved in the acquisition and spread of this gene in publicly available and complete genomes ofPseudomonasspp. AllPseudomonascomplete genomes were downloaded from NCBI's Refseq library and were inspected for the presence of thecrpPgene. The mobile elements carrying this gene were further characterized. ThecrpPgene was identified only inP. aeruginosa, in more than half of the complete chromosomes (61.9%,n= 133/215) belonging to 52 sequence types, of which the high-risk clone ST111 was the most frequent. We identified 136crpP-harboring integrative and conjugative elements (ICEs), with 93.4% belonging to the mating-pair formation G (MPFG) family. The ICEs were integrated at the end of a tRNA(Lys)gene and were all flanked by highly conserved 45-bp direct repeats. ThecrpP-carrying ICEs contain 26 core genes (2.2% of all 1193 genes found in all the ICEs together), which are present in 99% or more of thecrpP-harboring ICEs. The most frequently encoded traits on these ICEs include replication, transcription, intracellular trafficking and cell motility. Our work suggests that ICEs are the main vectors promoting the dissemination of the ciprofloxacin-modifyingcrpPgene inP. aeruginosa.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 10