Limosilactobacillus urinaemulieris sp. nov. and Limosilactobacillus portuensis sp. nov. isolated from urine of healthy women

• Type: Article in International Scientific Journal
• Date: 2021

Authors

Ksiezarek, M (Author) Other; Ribeiro, TG (Author) Other; Rocha, J (Author) Other; Grosso, F (Author) Other; Perovic, SU (Author) Other; Luisa Peixe (Author) FFUP

Journal

Title: International Journal of Systematic and Evolutionary Microbiology

Vol. 71

ISSN: 1466-5026

Publisher: Microbiology Society

Indexing

ISI Web of Knowledge - 0 Citations

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Other information

Authenticus ID: P-00T-YES

DOI: 10.1099/ijsem.0.004726

Abstract (EN): Two Gram-stain-positive strains, c9Ua_26_M-T and c11Ua_112_M-T, were isolated from voided urine samples from two healthy women. Comparative 16S rRNA gene sequences demonstrated that these novel strains were members of the genus Limosilactobacillus. Phylogenetic analysis based on pheS gene sequences and core genomes showed that each strain formed a separated branch and are closest to Limosilactobacillus vaginalis DSM 5837(T). The average nucleotide identity (ANI) and Genome-to-Genome Distance Calculator (GGDC) values between c9Ua_26_M-T and the closest relative DSM 5837(T) were 90.7 and 42.9%, respectively. The ANI and GGDC values between c11Ua_112_M-T and the closest relative DSM 5837(T) were 91.2 and 45.0%, and those among the strains were 92.9% and 51,0%, respectively. The major fatty acids were C-12:0 (40.2 %), C-16:0 (26.7%) and C-18:1 omega 9c (17.7%) for strain c9Ua_26_M-T, and C-18:1 omega 9c (38.0 %), C-16:0 (33.3%) and C-12:0 (17.6 %) for strain c11Ua_112_M-T. The genomic DNA G+C content of strains c9Ua_26_M-T and c11Ua_112_M-T was 39.9 and 39.7 mol%, respectively. On the basis of the data presented here, strains c9Ua_26_M-T and c11Ua_112_M-T represent two novel species of the genus Limosilactobacillus, for which the names Limosilactobacillus urinaemulieris sp. nov. (c9Ua_26_M-T=CECT 30144(T)=LMG 31899(T)) and Limosilactobacillus portuensis sp. nov. (c11Ua_112_M-T=CECT 30145(T)=LMG 31898(T)) are proposed.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 5